Investigation on the in vitro anti-Trichophyton activity of photosensitizers

Author(s)

da Silva, Ana Paula ; Uliana, Marciana Pierina ; Gontijo Guimaraes, Francisco Eduardo ; de Oliveira, Kleber Thiago ; Blanco, Kate Cristina ; Bagnato, Vanderlei Salvador ; Inada, Natalia Mayumi

• Document type: Journal article
• Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 21, n. 7, p. 8-pg., 2022-03-24.

Abstract

Onychomycosis is the most common disease caused by fungal nail infections, and often caused by dermatophytes. This infection is very resistant to antifungal treatments, and promising Photodynamic Therapy (PDT) mediated treatments has been presented as a multitarget tracking. Optimization of PDT guide for uptake time, concentration of photosensitizers (PS) and the light dose to inactivate Trichophyton mentagrophytes. Curcumin derivatives, porphyrin Chlorin e6 (CHL-E6) and Chlorin-P6-6-N-butylamide-7-methyl-ester (CHL-butyl) were evaluated. PS photobleaching was observed on the hyphae photosensitized over the time, correlating the PS concentration and light dose of antifungal PDT. Porphyrin, Curcumin, Chl-e6 and Chl-butyl concentrations of 2.5 mu g/mL, 0.025 mu g/mL, 10 mu g/mL and 5 mu g/mL respectively, under illumination of 10.5 J/cm(2) were the best antifungal conditions found in the study. Curcumin, in low concentrations, and chlorin were the PSs with higher activity anti-T. mentagrophytes. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/19720-8 - Synthesis and Photodynamic Activity of New photosensitizers derived from porphyrinoids natural products
• Grantee: Marciana Peirina Uliana
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13569-8 - Study of the Mechanisms of Action in Photodynamic Therapy: From Photosensitizer to Practical Application
• Grantee: Lucas Danilo Dias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/09952-0 - Treatment of pharyngotonsillitis with photodynamic action: investigation of basic mechanisms
• Grantee: Kate Cristina Blanco
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor