In vitro evaluation of the cis-[Ru(phen)(2()pPDIp)](2+**) complex for antimicrobial photodynamic therapy against Sporothrix brasiliensis and Candida albicans

Author(s)

Tiburcio, M. A. ; Rocha, A. R. ; Romano, R. A. ; Inada, N. M. ; Bagnato, V. S. ; Carlos, R. M. ; Buzza, H. H.

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 229, p. 8-pg., 2022-03-09.

Abstract

Background: Photodynamic therapy (PDT) activates a photosensitizer by visible light to generate cytotoxic oxygen species that lead to cell death. With proper illumination, PDT is often used in applications on superficial and sub-surface lesions. Sporotrichosis infection occurs by Sporothrix fungi which causes a skin wound, worsened by Candida albicans infections. This study investigated the photosensitizing efficiency of the Ru(phen)2(pPDIp) (PF6)2 complex, RupPDIp, against S. brasiliensis and C. albicans.Material and methods: RupPDIp efficiency against these fungi was tested using 450 nm (blue light and 36 J/cm2) and 525 nm (green light, 25.2 J/cm2) at 0.05-20 mu M concentrations. To ensure PDT effectiveness, control groups were tested in the absence and in the presence of RupPDIp under light irradiation and in the dark. Results: RupPDIp eliminated both fungi at <= 5.0 mu M. Green light showed the best results, eliminating S. brasiliensis and C. albicans colonies at RupPDIp 0.5 mu M and 0.05 mu M, respectively.Conclusion: RupPDIp is a promising photosensitizer in aPDT, eliminating 106 CFU/mL of both fungi at 450 nm and 525 nm, with lower light doses and concentrations when treated with the green light compared to the blue light. (AU)

• FAPESP's process: 18/03424-0 - Ru(II)-perylenediimide complexes for activation of singlet oxygen: photodynamic therapy and cellular imaging for treatment of Melanoma Cancer
• Grantee: Isabele Aparecida Soares de Campos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/21143-0 - Amyloid protein aggregates and the relationship between Alzheimer's Disease and type 2 Diabetes investigated by Ru(II) luminescent complexes
• Grantee: Rose Maria Carlos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/14033-6 - Basic studies of the combination of photothermal and photodynamic effects in application of a new modality for the Cancer treatment
• Grantee: Hilde Harb Buzzá
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC