Differential reaction of sweet pepper to infection with the crinivirus tomato chlorosis virus probably depends on the viral variant

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Vicentin, Eduardo ; Mituti, Tatiana ; Nogueira, Angelica Maria ; Moura, Monika Fecury ; Bello, Vinicius Henrique ; Ribeiro-Junior, Marcos Roberto ; Wintermantel, William M. ; Fiallo-Olive, Elvira ; Navas-Castillo, Jesus ; Krause-Sakate, Renate ; Marques Rezende, Jorge Alberto

• Document type: Journal article
• Source: PLANT PATHOLOGY; v. 71, n. 6, p. 10-pg., 2022-05-05.

Abstract

The tomato chlorosis virus (ToCV), transmitted by whitefly species of the genera Bemisia and Trialeurodes in a semipersistent manner, causes significant losses in solanaceous crops including tomato (Solanum lycopersicum) and sweet pepper (Capsicum annuum). Worldwide reports of natural and experimental infection of sweet pepper plants with ToCV are contradictory, raising the question of whether the critical factor determining infection is related to the susceptibility of sweet pepper cultivars or the genetics of virus isolates. In this work, ToCV isolates obtained from different hosts and geographical origins were biologically and molecularly analysed, transmitted by B. tabaci MEAM1 and MED, and the reaction of different sweet pepper cultivars was evaluated under different environmental conditions. Brazilian ToCV isolates from tomato, potato (S. tuberosum), S. americanum, and Physalis angulata did not infect plants of five sweet pepper cultivars when transmitted by B. tabaci MEAM1. Temperatures did not affect the sweet pepper susceptibility to tomato-ToCV isolates from Sao Paulo, Brazil, and Florida, USA. However, sweet pepper-ToCV isolates from Spain and Sao Paulo, Brazil, were transmitted efficiently to sweet pepper plants by B. tabaci MEAM1 and MED. Although the results indicated that ToCV isolates from naturally infected sweet pepper plants seem to be better adapted to plants of C. annuum, phylogenetic analyses based on the complete nucleotide sequences of RNA1 and RNA2 as well as the p22 gene did not reveal significant nucleotide differences among them. Additional studies are needed to identify intrinsic characteristics of ToCV isolates that favour infection of sweet pepper plants. (AU)

• FAPESP's process: 19/18639-4 - Interactions involving Begomovirus, Solanaceae and different cryptic species of Bemisia tabaci
• Grantee: Angélica Maria Nogueira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/21588-7 - Bemisia tabaci in Brazil: competitive displacement between criptic species, susceptibility to insecticides, next generation sequencing analysis, virus influence on insect biology and the presence of the endosymbiont Hamiltonella in virus transmission
• Grantee: Renate Krause Sakate
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18274-3 - Begomovirus and crinivirus in solanasceous: regional molecular epidemiology and sustainable integrated management alternatives
• Grantee: Jorge Alberto Marques Rezende
• Support Opportunities: Research Projects - Thematic Grants