| Full text | |
| Author(s): Show less - |
Lazarini, Josy Goldoni
;
Massarioli, Adna Prado
;
Soares, Jackeline Cintra
;
Nani, Bruno Dias
;
Charo, Nancy
;
Oliveira, Douglas Souza
;
Camargo, Lauren
;
Alvarez-Flores, Miryam Paola
;
Correia Batista, Isabel de Fatima
;
Chudzinski-Tavassi, Ana Marisa
;
de Alencar, Severino Matias
;
Franchin, Marcelo
;
Rosalen, Pedro Luiz
Total Authors: 13
|
| Document type: | Journal article |
| Source: | FOOD CHEMISTRY: MOLECULAR SCIENCES; v. 4, p. 10-pg., 2022-03-09. |
| Abstract | |
We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-kappa B activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11 b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-kappa B activation, IL-1 beta, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies. (AU) | |
| FAPESP's process: | 17/09898-0 - Bioprospection of novel anti-inflammatory molecules from natural Brazilian native products |
| Grantee: | Pedro Luiz Rosalen |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/02926-6 - Phytochemistry and bioprospection of antioxidant and anti-inflammatory activities from unexplored Brasilian native fruits. |
| Grantee: | Josy Goldoni Lazarini |
| Support Opportunities: | Scholarships in Brazil - Doctorate |