Biological activity of pectic polysaccharides investigated through biomembrane models formed at the air-water interface

Author(s)

Escobar, Bruno de Andrade ; Valerio, Gabriel Lundgren Ferreira ; Caseli, Luciano

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 216, p. 6-pg., 2022-05-12.

Abstract

Pectin, a polysaccharide with potential bioactivity, was inserted in the aqueous subphase of monolayers of selected lipids DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and DPPE (1,2-dipalmitoyl-sn-glycero-3phosphoethanolamine), representing mammalian and bacterial membranes, respectively. Pectin condensed monolayers but made the DPPC monolayer more fluid, while for DPPE, it made its monolayer more rigid, detected with dynamic interfacial rheology. Complementary data using surface potential, infrared spectroscopy, and Brewster angle microscopy also showed distinctive effects of pectin on DPPE and DPPC. We believe these data can be correlated with the action of this polysaccharide with biological lipidic surfaces with different polar heads, which may be relevant, generally speaking, to understanding the molecular mechanism of this bioactive compound for pharmaceutical purposes. (AU)

• FAPESP's process: 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants