Amino acid decorated xanthan gum coatings: Molecular arrangement and cell adhesion

Author(s)

Alavarse, Alex Carvalho ; Frachini, Emilli Caroline G. ; Silva, Jean Bezerra ; Pereira, Rafael dos Santos ; Ulrich, Henning ; Siqueira Petri, Denise Freitas

• Document type: Journal article
• Source: CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS; v. 4, p. 11-pg., 2022-06-13.

Abstract

In this work, citric acid molecules mediated simultaneously the crosslinking of xanthan gum (XG) chains and the grafting of glutamic acid (Glu), cysteine (Cys), histidine (His) or tryptophan (Trp) to XG chains, as evidenced by Fourier-transform infrared spectroscopy, elemental and thermogravimetric analyses, and high-resolution X-ray photoelectron spectroscopy. XG coatings (similar to 60 nm thick) presented total surface energy (gamma S) of 65 mJ/m(2). The attachment of amino acids to the XG chains decreased.S values to similar to 48 mJ/m(2) because the surface energy polar (gamma S-p) component decreased. Circular dichroism (CD) spectra revealed that the helix conformation of XG chains was retained in XG and XG-Glu-coatings, but it was lost in the XG-His, XG-Cys-and XG-Trp-coatings due to intermolecular interactions between the amino acid side groups (thiol, imidazole, and indole). XG-based coatings presented no cytotoxicity. After 3 h of incubation, the adhesion of SH-5YSY neural cells in comparison to the control (100%) was XG-His (18%), XG-Cys (21.4%), XG (29%), XG-Trp (28.6%) and XG-Glu (46.4%). The decrease of% cell adhesion tended to be favored by the decrease of gamma S-p, except for XG-Glu-and XG-Trp, and by the loss of helix molecular conformation of chains in the coatings. (AU)

• FAPESP's process: 20/01907-3 - Determination of mechanical properties of polysaccharide-based magnetic scaffolds
• Grantee: Alex Carvalho Alavarse
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/07366-4 - Purine and kinin receptors as targets of study and therapeutic interventions in neurological diseases
• Grantee: Alexander Henning Ulrich
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants