Antimicrobial action of four herbal plants over mixed-species biofilms of Candida albicans with four different microorganisms

Author(s)

Domingues, Nadia ; Ramos, Lucas de Paula ; Pereira, Larissa Marques ; do Rosario Estevam dos Santos, Pamela Beatriz ; Scorzoni, Liliana ; Pereira, Thais Cristine ; Abu Hasna, Amjad ; Talge Carvalho, Claudio Antonio ; de Oliveira, Luciane Dias

• Document type: Journal article
• Source: AUSTRALIAN ENDODONTIC JOURNAL; v. N/A, p. 10-pg., 2022-09-04.

Abstract

This study aimed to evaluate the antimicrobial effect of four herbal plants glycolic extracts over mixed-species biofilm composed of Candida albicans (C. albicans) and another pathogenic bacterium as alternative therapy to be investigated. Four plants extract of Pfaffia paniculata roots; Hamamelis virginiana leaf, Stryphnodendron barbatiman tree bark and Gymnema sylvestre stem and leaves were tested over multi-species biofilm of C. albicans (ATCC 18804) and Streptococcus mutans (ATCC 35688), Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 4083) or Pseudomonas aeruginosa (ATCC 15442) for 5 min and 24 h and colony forming units per millilitre was calculated. The data were analysed using Kruskal-Wallis with Dunn's test (p <= 0.05). All tested extracts showed antimicrobial action over the mixed-species biofilms after 24 h. Some extracts eliminated totally the biofilms. The glycolic extract of P. paniculata, H. virginiana, S. barbatiman and G. sylvestre are effective over mixed-species biofilms and may be indicated as endodontic irrigant or intracanal medication. (AU)

• FAPESP's process: 17/26941-7 - Antimicrobial action of P. paniculata glycolic extract on Candida albicans biofilms associated with different bacteria
• Grantee: Larissa Marques Pereira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/08776-3 - ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY, CYTOTOXICITY AND GENOTOXICITY OF DIFFERENT NATURAL EXTRACTS
• Grantee: Luciane Dias de Oliveira
• Support Opportunities: Regular Research Grants