Exploratory lipidome and metabolome profiling contributes to understanding differences in high and normal ultimate pH beef

Author(s):Show less -

Antonelo, Daniel S. ; dos Santos-Donado, Priscila R. ; Ferreira, Christina R. ; Colnago, Luiz A. ; Ocampos, Fernanda M. M. ; Ribeiro, Gabriel H. ; V. Ventura, Ricardo ; Gerrard, David E. ; Delgado, Eduardo F. ; Contreras-Castillo, Carmen J. ; Balieiro, Julio C. C.

• Document type: Journal article
• Source: MEAT SCIENCE; v. 194, p. 11-pg., 2022-12-01.

Abstract

The aim of this work was to compare the lipidome and metabolome profiling in the Longissimus thoracis muscle early and late postmortem from high and normal ultimate pH (pHu) beef. Lipid profiling discriminated between high and normal pHu beef based on fatty acid metabolism and mitochondrial beta-oxidation of long chain saturated fatty acids at 30 min postmortem, and phospholipid biosynthesis at 44 h postmortem. Metabolite profiling also discriminated between high and normal pHu beef, mainly through glutathione, purine, arginine and proline, and glycine, serine and threonine metabolisms at 30 min postmortem, and glycolysis, TCA cycle, glutathione, tyrosine, and pyruvate metabolisms at 44 h postmortem. Lipid and metabolite profiles showed reduced glycolysis and increased use of alternative energy metabolic processes that were central to differenti-ating high and normal pHu beef. Phospholipid biosynthesis modification suggested high pHu beef experienced greater oxidative stress. (AU)

• FAPESP's process: 19/18346-7 - Beef quality in Bos indicus cattle - biological markers for meat attributes in different ultimate pH ranges
• Grantee: Priscila Robertina dos Santos Donado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/26667-2 - Beef quality in Bos indicus cattle: biological markers for meat attributes in different ultimate pH ranges
• Grantee: Carmen Josefina Contreras Castillo
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/26378-3 - Correlation of the muscle proteome with meat quality from Angus x Nellore cattle finished in different finishing systems, weight gain rates, and slaughter criterion
• Grantee: Júlio Cesar de Carvalho Balieiro
• Support Opportunities: Regular Research Grants