Determination of selected herbicides in sugarcane-derived foods by graphene-oxide based disposable pipette extraction followed by liquid chromatography-tandem mass spectrometry

Author(s)

Oliveira, Thais Camarotto ; Lancas, Fernando Mauro

• Document type: Journal article
• Source: Journal of Chromatography A; v. 1687, p. 11-pg., 2023-01-04.

Abstract

Sugarcane is widely cultivated in Brazil. Although there are Maximum Residue Limits of pesticides deter-mined for this plant, there is no legislation covering alimentary products from sugarcane. In this study, Disposable Pipette Tip Extraction (DPX) technique was evaluated as a sample preparation technique for simultaneous determination of eleven herbicides followed by LC-MS/MS analysis in three sugarcane -derived food matrices: juice, candy, and syrup. First, graphene oxide anchored to silica functionalized with octadecyl silane and endcapped was synthesized, which was evaluated as a sorbent in DPX. Then, after evaluating the parameters involved in DPX extraction, the method was validated following the ICH guide. As a result, the method showed acceptable linearity ( r >= 0.99), limits of quantification (1.0 - 5.0 ng mL (-1) for juice and 5.0 - 25.0 ng g( - 1) for candy and syrup, varying according to the pesticide), precision, and accuracy within the limits of the literature, and recoveries ranging from 48 - 69% (juice), 34 - 89% (candy), and 28 - 76% (syrup). Finally, the developed method was successfully applied in actual samples of the three studied matrices. (C) 2022 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry
• Grantee: Deyber Arley Vargas Medina
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/07347-9 - Development of novel materials and devices for miniaturized sample preparation techniques in the area of food safety
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/15462-5 - Employment of graphene as sorbent in miniaturized sample preparation techniques.
• Grantee: Bruno Henrique Fumes
• Support Opportunities: Scholarships in Brazil - Doctorate