In Vivo and In Silico Study of Photodynamic Necrosis Volume in Rat Liver

Author(s)

Garcia, Marlon Rodrigues ; Sanchez, Victor ; Fortunato, Thereza Cury ; Requena, Michelle Barreto ; Grecco, Clovis ; Vollet-Filho, Jose Dirceu ; Pires, Layla ; Moriyama, Lilian Tan ; Pratavieira, Sebastiao

• Document type: Journal article
• Source: PHOTONICS; v. 9, n. 12, p. 12-pg., 2022-12-01.

Abstract

Photodynamic therapy is a treatment modality that can be used to treat various types of lesions. To produce cell death, reaching a certain threshold dose of reactive oxygen species (ROS) is required. The estimation of ROS production is of paramount importance to predict the depth of necrosis and to ensure that the volume to be treated receives doses higher than the threshold. In this study, we compared a theoretical model for PDT based on Monte Carlo simulations of light irradiance and rate equations with a rat liver model. At the end of the simulation, necrosis depths and volumes were estimated, as well as the photosensitizer (PS), oxygen, and ROS concentrations at each position of the treated area. From the in vivo study, we obtained the ROS concentration threshold of about 1 mM for Photogem in rat liver. This proposed method can be used for any PS or tissue, including tissues with multiple layers. The proposed method can be used to estimate parameters for any PS or tissue, including layered tissues, as long as their parameters are known. In addition, other protocols can be tested, or compared with the standard ones, providing the bases for analyzing a diverse range of photodynamic treatment scenarios. (AU)

• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/10860-6 - USE OF DISSOLVING MICRONEEDLES CONTAINING AMINOLEVULINIC ACID FOR PHOTODYNAMIC THERAPY
• Grantee: Michelle Barreto Requena
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC