Development of multiparticulate systems based on natural polymers for morin controlled release

Author(s)

Sales, Luciana Solera ; Gimenes, Milena da Silva ; Meneguin, Andreia Bagliotti ; Barud, Hernane da Silva ; Achcar, Jorge Alberto ; Brighenti, Fernanda Lourencao

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 228, p. 12-pg., 2022-12-23.

Abstract

This study aimed to develop a multiparticulate system based on sodium alginate/gellan gum polymers for morin controlled release using standardized spray-dryer parameters. A 24 experimental factorial design was used to standardize spray-dryer parameters. After standardization, three systems with three different proportions of the natural polymers (50:50, 25:75, 75:25; sodium alginate: gellan gum) with and without morin (control) were developed. The systems were characterized according to its morphology and physicochemical properties. Next, the systems were evaluated regarding antibiofilm and antimicrobial activity against Streptococcus mutans. The factorial design indicated the use of the following parameters: i) air flow rate: 1.0 m3 /min; ii) outlet temper-ature: 120 degrees C; iii) natural polymers combination in different proportions; iiii) polymer concentration: 2 %. Scanning electron microscopy showed microparticles with spherical shape and rough surface. The samples released 99.86 % +/- 9.36; 85.45 % +/- 8.31; 86.87 % +/- 3.83 of morin after 480 min. The systems containing morin significantly reduced S. mutans biofilm biomass, microbial viability and acidogenicity when compared to their respective controls. In conclusion, the spray-dryer parameters were standardized to the highest possible yield values and proved to be efficient for morin encapsulation and controlled release. Furthermore, these systems controlled important virulence factors of S. mutans biofilms. (AU)

• FAPESP's process: 19/26066-4 - Mucoadhesive multiparticulate system for controlled release of natural substances
• Grantee: Luciana Solera Sales
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/19817-3 - Bioresponsive systems based on retrograded starch/pectin containing cellulose nanofibers as a strategy for 5-ASA colonic delivery in the treatment of Inflammatory Bowel Disease
• Grantee: Andréia Bagliotti Meneguin
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico
• Grantee: Ana Eugênia de Carvalho Campos
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 21/12931-5 - Anti-inflammatory, antioxidant and antimicrobial evaluation of mucoadhesive multiparticulate system containing botanical substance
• Grantee: Luciana Solera Sales
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 19/08375-0 - Maximization of the anti-caries and antibiofilm effect of conventional substances: evaluation in a polymicrobial biofilm model
• Grantee: Fernanda Lourenção Brighenti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC