Wearable potentiometric biosensor for analysis of urea in sweat

Author(s)

Ibanez-Redin, Gisela ; Cagnani, Giovana Rosso ; Gomes, Nathalia O. ; Raymundo-Pereira, Paulo A. ; Machado, Sergio A. S. ; Gutierrez, Marco Antonio ; Krieger, Jose Eduardo ; Oliveira, Osvaldo N.

• Document type: Journal article
• Source: BIOSENSORS & BIOELECTRONICS; v. 223, p. 8-pg., 2022-12-26.

Abstract

Herein, we introduce wearable potentiometric biosensors on screen-printed carbon electrodes (SPCEs) for onbody and on-site monitoring of urea in sweat. The biosensor architecture was judiciously designed to detect urea at different pHs and incorporate a pH sensor, thus containing polyaniline ink, urease bioink and a polyvinylchloride membrane. Urea detection could be performed in the wide range from 5 to 200 mM at pH 7.0, encompassing urea levels in human sweat. The biosensor response was fast (incubation time 5 min), with no interference from other substances in sweat. Reliable urea detection could be done in undiluted human sweat with a skin-worn flexible device using the pH correction strategy afforded by the pH sensor. The performance of the epidermal biosensor was not affected by severe bending strains. The feasibility of mass production was demonstrated by fabricating epidermal flexible biosensors using slot-die coating with a roll-to-roll technique. (AU)

• FAPESP's process: 14/50889-7 - National Institute of Science and Technology Medicine Assisted by Scientific Computing (INCT-MACC)
• Grantee: José Eduardo Krieger
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/02164-0 - Development of electrochemical sensors modified with hollow carbon nanospheres doped with sulfur and nitrogen for the quantification of drugs in supply water samples
• Grantee: Sergio Antonio Spinola Machado
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/09587-8 - Development of electrochemical devices based on carbon and silver nanostructures to detect endocrine disruptors in quantities of water supply
• Grantee: Nathalia Oezau Gomes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection
• Grantee: Paulo Augusto Raymundo Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/14906-5 - Implementation of flexible sensors for diagnosis with data processing using information visualization and artificial intelligence
• Grantee: Glenda Gisela Ibanez Redin
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training