| Full text | |
| Author(s): Show less - |
Roque-Borda, Cesar Augusto
;
Saraiva, Mauro de Mesquita Souza
;
Junior, Wagner Dias Macedo
;
Montesinos, Jose Carlos Estanislao Marquez
;
Meneguin, Andreia Bagliotti
;
Borges, Anna Beatriz Toledo
;
Junior, Edson Crusca
;
Garrido, Saulo Santesso
;
Almeida, Adriana Maria de
;
Marchetto, Reinaldo
;
Chorilli, Marlus
;
Junior, Angelo Berchieri
;
Teixeira, Silvio Rainho
;
Pavan, Fernando Rogerio
;
Vicente, Eduardo Festozo
Total Authors: 15
|
| Document type: | Journal article |
| Source: | Biomaterials; v. 293, p. 15-pg., 2022-12-27. |
| Abstract | |
The incorrect use of conventional drugs for both prevention and control of intestinal infections has contributed to a significant spread of bacterial resistance. In this way, studies that promote their replacement are a priority. In the last decade, the use of antimicrobial peptides (AMP), especially Ctx(Ile21)-Ha AMP, has gained strength, demonstrating efficient antimicrobial activity (AA) against pathogens, including multidrug-resistant bacteria. However, gastrointestinal degradation does not allow its direct oral application. In this research, double-coating systems using alginate microparticles loaded with Ctx(Ile21)-Ha peptide were designed, and in vitro release assays simulating the gastrointestinal tract were evaluated. Also, the AA against Salmonella spp. and Escherichia coli was examined. The results showed the physicochemical stability of Ctx(Ile21)-Ha peptide in the system and its potent antimicrobial activity. In addition, the combination of HPMCAS and chitosan as a gastric protection system can be promising for peptide carriers or other low pH-sensitive molecules, adequately released in the intestine. In conclusion, the coated systems employed in this study can improve the formulation of new foods or biopharmaceutical products for specific application against intestinal pathogens in animal production or, possibly, in the near future, in human health. (AU) | |
| FAPESP's process: | 18/25707-3 - Application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in laying hens |
| Grantee: | Cesar Augusto Roque Borda |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 20/16573-3 - In vitro and in vivo studies of antimicrobial peptide B1CTcu5 analogs encapsulated in colon-specific microparticles against Mycobacterium tuberculosis |
| Grantee: | Cesar Augusto Roque Borda |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition |
| Grantee: | Eduardo Festozo Vicente |
| Support Opportunities: | Research Grants - Young Investigators Grants |