A phytotherapic blend immunity-6TM inhibits myeloid leukemic cells 2 activation involving purinergic signaling

Author(s)

Bella, Yanesko Fernandes ; Oliveira, Carlos Rocha ; Mateus-Silva, Jose Roberto ; Brandao-Rangel, Maysa Alves Rodrigues ; Silva-Reis, Anamei ; Santos, Juliana de Melo Batista ; Albertini, Regiane ; Lopes-Martins, Rodrigo Alvaro Brandao ; de Oliveira, Luis Vicente Franco ; Vieira, Rodolfo P.

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 159, p. 6-pg., 2023-01-16.

Abstract

Leukemia is among the most common types of hematological cancers and the use of herbal medicines to prevent and treat leukemia are under quick development. Among several molecular pathways involved in leukemia pathogenesis and exacerbations, purinergic signaling is revealed as a key component. In the present study, the effects of two doses (5 ug/mL and 10 ug/mL) of Immunity-6TM, a phytocomplex composed by beta-glucan, green tea (Camelia sinensis), chamomile (Matricaria chamomilla), and ascorbic acid (vitamin C) was tested in vitro, using chronic myelogenous leukemia cell line (K-562; 5 x104/mL/well), which were challenged with lipo-polysaccharide (LPS; 1 ug/mL) for 24 h. The results demonstrated that both doses of Immunity-6TM inhibited ATP release (p < 0.001) and P2x7 receptor at mRNA levels expression (p < 0.001). Purinergic inhibition by Im-munity-6TM was followed by reduced release of proinflammatory cytokines IL-1beta (p < 0.001) and IL-6 (p < 0.001), while only 5 ug/mL of Immunity-6TM reduced the release of TNF-alpha (p < 0.001). Beyond to inhibit the release of pro-inflammatory cytokines, both doses of Immunity-6TM induced the release of anti-inflammatory cytokine IL-10 (p < 0.001), while only the higher dose (10 ug/mL) of Immunity-6TM induced the release of anti-inflammatory IL-1ra (p < 0.05) and klotho (p < 0.001). Thus, Immunity-6TM may be a promising adjuvant in the treatment of leukemia and further clinical trials are guaranteed. (AU)

• FAPESP's process: 19/05739-0 - Role of kinins in the anti-inflammatory effects of aerobic training in experimental models of Asthma
• Grantee: Maysa Alves Rodrigues Brandão Rangel
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/11244-4 - Effects of combined physical training on the pulmonary mechanics and function and on the pulmonary and systemic immune response of obese women
• Grantee: Anamei Silva Reis
• Support Opportunities: Scholarships in Brazil - Master