| Full text | |
| Author(s): Show less - |
Sousa, Thiago F. F.
;
de Araujo Junior, Moyses B.
;
Peres, Eldrinei G. G.
;
Souza, Mayane P. P.
;
da Silva, Felipe M. A.
;
de Medeiros, Livia S.
;
de Souza, Afonso D. L.
;
de Souza, Antonia Q. L.
;
Yamagishi, Michel E. B.
;
da Silva, Gilvan F. F.
;
Koolen, Hector H. F.
;
De Queiroz, Marisa V. V.
Total Authors: 12
|
| Document type: | Journal article |
| Source: | Archives of Microbiology; v. 205, n. 2, p. 12-pg., 2023-02-01. |
| Abstract | |
Fungi of the genus Penicillium section Sclerotiora have as their main characteristic the presence of orange-pigmented mycelium, which is associated with sclerotiorin, a chlorinated secondary metabolite of the azaphilone subclass of polyketides. Sclerotiorin presents anti-diabetes, antioxidant, anti-inflammatory, anti-Alzheimer, antiviral, and antimicrobial activities, which has always attracted the attention of researchers worldwide. During our ongoing search for azaphilone-producing Amazonian fungi, the strain of Penicillium MMSRG-058 was isolated as an endophyte from the roots of Duguetia stelechantha and showed great capacity for producing sclerotiorin-like metabolites. Using multilocus phylogeny, this strain was identified as Penicillium meliponae. Moreover, based on the genome mining of this strain through the reverse approach, a cluster of putative biosynthetic genes (BGC) responsible for the biosynthesis of sclerotiorin-like metabolites (scl cluster) was identified. The knockout of the sclA (highly reducing PKS) and sclI (non-reducing PKS) genes resulted in mutants with loss of mycelial pigmentation and terminated the biosynthesis of sclerotiorin-like metabolites: geumsanol B, chlorogeumsanol B, 7-deacetylisochromophilone VI, isochromophilone VI, ochrephilone, isorotiorin, and sclerotiorin. Based on these results, a biosynthetic pathway was proposed considering the homology of BGC scl genes with the azaphilone BGCs that have already been functionally characterized. (AU) | |
| FAPESP's process: | 20/08270-0 - Metabolic diversification of Penicillium fungi isolated from the Atlantic Forest and Amazon biomes: a perspective for biomolecules production |
| Grantee: | Lívia Soman de Medeiros |
| Support Opportunities: | Regular Research Grants |