| Full text | |
| Author(s): Show less - |
de Souza Araujo, Isaac Jordao
;
Ricardo, Mariana Gallante
;
Gomes, Orisson Ponce
;
Giovani, Priscila Alves
;
Puppin-Rontani, Julia
;
Pecorari, Vanessa Arias
;
Martinez, Elizabeth Ferreira
;
Napimoga, Marcelo Henrique
;
Nociti Junior, Francisco Humberto
;
Puppin-Rontani, Regina Maria
;
Lisboa-Filho, Paulo Noronha
;
Kantovitz, Kamila Rosamilia
Total Authors: 12
|
| Document type: | Journal article |
| Source: | BRAZILIAN ORAL RESEARCH; v. 35, p. 12-pg., 2021-01-01. |
| Abstract | |
This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix (R) at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey's and Dunn's post-hoc tests (c =0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC. (AU) | |
| FAPESP's process: | 16/13786-0 - Effect of Novel Nanotechnology to Conventional Glass Ionomer Cement on its Physical-Chemical-Biological Properties |
| Grantee: | Kamila Rosamilia Kantovitz |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/14078-8 - Effect of titanium dioxide nanotubes on biological properties of conventional glass ionomer cement |
| Grantee: | Kamila Rosamilia Kantovitz |
| Support Opportunities: | Regular Research Grants |