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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections

Full text
Author(s):
Cristiane DUQUE ; Amanda Caselato Andolfatto SOUZA [2] ; Kelly Limi AIDA [3] ; Jesse Augusto PEREIRA [4] ; Karina Sampaio CAIAFFA [5] ; Vanessa Rodrigues dos SANTOS [6] ; Leopoldo COSME-SILVA [7] ; Anuradha PRAKKI [8]
Total Authors: 8
Document type: Journal article
Source: Journal of Applied Oral Science; v. 31, 2023-03-27.
Abstract

Abstract Objective This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. Methodology This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. Results The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. Conclusion EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity. (AU)

FAPESP's process: 17/10940-1 - Flavonoids combinations in thermosensitive hidrogels as proposals of endodontic medications for young permanent teeth
Grantee:Cristiane Duque
Support Opportunities: Regular Research Grants