Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia

Author(s)

Mateus Souza Abreu ^[1] ; Ana Cristina Aoun Tannuri ^[2] ; Rafael Felipe Gonçalves Rodrigues ^[3] ; Rafael José da Silva ^[4] ; Josiane de Oliveira Gonçalves ^[5] ; Suellen Serafini ^[6] ; Uenis Tannuri ^[7]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[5] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[6] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[7] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 38, 2023-06-02.

Abstract

ABSTRACT Purpose: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model. Methods: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses. Results: In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method. Conclusions: IPoC methods positively reduced the damage caused by IRI in weaning rats. (AU)

• FAPESP's process: 19/12465-4 - Histological and molecular evaluation of the effects of direct and remote ischemic postconditioning on acute intestinal ischemia in young rats
• Grantee: Mateus Souza Abreu
• Support Opportunities: Scholarships in Brazil - Scientific Initiation