| Full text | |
| Author(s): Show less - |
Pimenta, Ruan
;
Camargo, Juliana A.
;
Goncalves, Guilherme L.
;
Ghazarian, Vitoria
;
Candido, Patricia
;
Guimaraes, Vanessa R.
;
Romao, Poliana
;
Chiovatto, Caroline
;
da Silva, Karina Serafim
;
dos Santos, Gabriel A.
;
Silva, Iran A.
;
Nahas, William C.
;
Leite, Katia R.
;
Pessoa, Ana Flavia Marcal
;
Viana, Nayara I.
;
Reis, Sabrina T.
Total Authors: 16
|
| Document type: | Journal article |
| Source: | MOLECULAR BIOLOGY REPORTS; v. 50, n. 9, p. 13-pg., 2023-07-13. |
| Abstract | |
BackgroundPreviously, we demonstrated that cholesterol triggers the increase in p300/CBP-associated factor (PCAF), targeted by miR-17-5p. The p300, IL-6, PCAF, and miR-17-5p genes have important and contradictory roles in inflammation and prostate cancer (PCa). This study aimed to demonstrate the potential anti-inflammatory effect of miR-17-5 in an advanced PCa model with diet-induced hypercholesterolemia.Methods and resultsIn vitro, using the PC-3 cell line, we show that induction of miR-17-5p reduces p300 and PCAF expression, increases apoptosis, and decreases cell migration. Furthermore, we demonstrate that supplementing this same cell with cholesterol (2 & mu;g/mL) triggers increased p300, IL-6, and PCAF. In vivo, after establishing the hypercholesterolemic (HCOL) model, xenografts were treated with miR-17-5p. Increased expression of this miR after intratumoral injections attenuated tumor growth in the control and HCOL animals and reduced cell proliferation.ConclusionOur results demonstrate that inducing miR-17-5p expression suppresses tumor growth and inflammatory mediator expression. Further studies should be conducted to fully explore the role of miR-17-5p and the involvement of inflammatory mediators p300, PCAF, and IL-6. (AU) | |
| FAPESP's process: | 18/26528-5 - Adaptive mechanisms of renal function in a model of adriamycin-induced nephrotoxicity: participation of sirtuin 1 and claudin-1 |
| Grantee: | Guilherme Lopes Gonçalves |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/19906-3 - Genomic edition with CRISPR/Cas9 to evaluate the role of MMP9 and the regulator miR21 in Prostate Cancer |
| Grantee: | Juliana Alves de Camargo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 19/19138-9 - Study of the effect of miR-137 on cell line of castration resistant prostate cancer in a hypercholesterolemic environment |
| Grantee: | Vitória Ghazarian Nunes |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 22/09284-0 - Searching for alterations in the expression of mismatch-type repair proteins (MLH1, MSH2, MSH6, and PMS2) in Prostate Cancer |
| Grantee: | Karina Serafim da Silva |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 19/00156-7 - In vivo study of co-activators and co-repressors of androgen receptor in Prostate Câncer |
| Grantee: | Ruan César Aparecido Pimenta |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 21/02341-6 - Establishment of the animal model of Peyronie's disease fibrin-induced |
| Grantee: | Caroline Brandão Chiovatto |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 20/01317-1 - Evaluation of the role of microRNAs -17-5p and -137 on androgen receptor cofactors in castration-resistant prostate cancer: in vitro and in vivo study in a hypercholesterolemic model |
| Grantee: | Sabrina Thalita dos Reis Faria |
| Support Opportunities: | Regular Research Grants |