NanoLC-EI-MS: Perspectives in Biochemical Analysis

Author(s)

dos Santos, Natalia Gabrielly Pereira ; Maciel, Edvaldo Vasconcelos Soares ; Medina, Deyber Arley Vargas ; Lancas, Fernando Mauro

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 14, p. 14-pg., 2023-07-01.

Abstract

Although LC-MS with atmospheric pressure ionization (API) sources is the primary technique used in modern bioanalytical studies, electron ionization mass spectrometry (EI-MS) can provide some substantial advantages over it. EI-MS is a matrix effect-free technique that provides reproducible and comparable mass spectra, serving as a compound fingerprint for easy identification through automated comparison with spectral libraries. Leveraging EI-MS in biochemical studies can yield critical analytical benefits for targeted and untargeted analyses. However, to fully utilize EI-MS for heavy and non-volatile molecules, a new technology that enables the coupling of liquid chromatography with EI-MS is needed. Recent advancements in nanoLC have addressed the compatibility issues between LC and EI-MS, and innovative interfacing strategies such as Direct-EI, liquid electron ionization (LEI), and Cold-EI have extended the application of EI-MS beyond the determination of volatile organic molecules. This review provides an overview of the latest developments in nanoLC-EI-MS interfacing technologies, discussing their scope and limitations. Additionally, selected examples of nanoLC-EI-MS applications in the field of biochemical analysis are presented, highlighting the potential prospects and benefits that the establishment of this technique can bring to this field. (AU)

• FAPESP's process: 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry
• Grantee: Deyber Arley Vargas Medina
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/21984-0 - Multi-user equipment approved in grant 17/02147-0: electron ionization nano liquid chromatography-mass spectrometry system with a quadrupole analyzer (EI-Q (MS)) - nano-LC-EI-MS
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/21985-6 - Multi-user equipment approved in grant 17/02147-0: electrospray ionization nano/micro liquid chromatography-tandem mass spectrometry system (ESI-QqQ (MS/MS)) - nano/µ-LC-ESI-MS/MS
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 19/26263-4 - Development of open tubular capillary columns for micro and nano liquid chromatography coupled to sequential mass spectroscopy
• Grantee: Natalia Gabrielly Pereira dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)