Infection of groundnut ringspot virus in Plumeria pudica characterized by irregular virus distribution and intermittent expression of symptoms

Author(s)

Favara, Gabriel Madoglio ; de Oliveira, Felipe Franco ; Ferro, Camila Geovana ; Kraide, Heron Delgado ; Carmo, Eike Yudi Nishimura ; Bello, Vinicius Henrique ; Ribeiro-Junior, Marcos Roberto ; Krause-Sakate, Renate ; Kitajima, Elliot Watanabe ; Rezende, Jorge Alberto Marques

• Document type: Journal article
• Source: FRONTIERS IN PLANT SCIENCE; v. 14, p. 12-pg., 2023-07-26.

Abstract

Plumeria pudica, known as bridal bouquet, exhibiting characteristic symptoms of orthotospovirus infection were found in different localities in Brazil. Symptoms were restricted to leaves of the middle and lower thirds of a few branches of each plant. Electron microscopy, molecular analyses, and complete genome sequencing identified the orthotospovirus as groundnut ringspot virus (GRSV),member of the species Orthotospovirus arachianuli. The virus was poorly transmitted mechanically to P. pudica. Reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analyses performed using total RNA extracted from leaf blades, primary veins, petioles, and regions of petiole insertion on branches indicated the presence of GRSV, predominantly in the symptomatic leaf blades. Symptomatic branches propagate vegetatively, often resulting in plants expressing GRSV symptoms. In contrast, vegetative propagation of the asymptomatic branches of infected plants predominantly generates plants without GRSV symptoms. The resistance of P. pudica plants to GRSV infection, restricted systemic viral movement, and expression of symptoms in infected plants suggest that this orthotospovirus does not threaten this ornamental plant. (AU)

• FAPESP's process: 18/01633-0 - Tomato severe rugose virus: potential of susceptible species as source of virus inoculum, vertical and horizontal transmission by B. tabaci MEAM1 and selection of mild strains for pre-immunization in tomato
• Grantee: Gabriel Madoglio Favara
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/02179-4 - Evaluation of the diversity of molecular pathogens (virus and viroids) of plants in Brazi: Phase III - 2021/2023
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 18/18274-3 - Begomovirus and crinivirus in solanasceous: regional molecular epidemiology and sustainable integrated management alternatives
• Grantee: Jorge Alberto Marques Rezende
• Support Opportunities: Research Projects - Thematic Grants