Whole genome sequencing of Bacillus anthracis isolated from animal in the 1960s, Brazil, belonging to the South America subclade

Author(s)

de Andrade, Tania Sueli ; Camargo, Carlos Henrique ; Campos, Karoline Rodrigues ; Reis, Alex Domingos ; Santos, Marlon Benedito do Nascimento ; Zanelatto, Vanessa Nieri ; Takagi, Elizabeth Harummyy ; Sacchi, Claudio Tavares

• Document type: Journal article
• Source: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 100, p. 6-pg., 2023-09-01.

Abstract

Bacillus anthracis causes anthrax disease and can affect humans and other animals. This zoonotic disease has an impact on the economic and health aspects. B. anthracis population is divided into three major clades: A (with worldwide distribution), B, and C (restricted to specific regions). Anthrax is most common in agricultural regions of central and southwestern Asia, sub-Saharan Africa, Southern and Eastern Europe, the Caribbean, and Central and South America. Here, we sequenced by short and long reads technologies to generate a hybrid assembly of a lineage of B. anthracis recovered from animal source in the 1960s in Brazil. Isolate identification was confirmed by phenotypic/biochemical tests and MALDI-TOF MS. Antimicrobial susceptibility was performed by in-house broth microdilution. B. anthracis IAL52 was susceptible to penicillin, amoxicillin, doxycycline, levofloxacin, and tetracycline but non-susceptible to ciprofloxacin. IAL52 was classified as sequence type ST2, clade A.Br.069 (V770 group). Sequencing lineages of B. anthracis, especially from underrepresented regions, can help determine the evolution of this critical zoonotic and virulent pathogen. (AU)

• FAPESP's process: 18/21192-9 - Multi-User Equipment approved in grant 2017/50333-7: MALDI-TOF
• Grantee: Carlos Henrique Camargo
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 17/50333-7 - Institutional research development plan of the Instituto Adolfo Lutz (PDIp)
• Grantee: Carlos Henrique Camargo
• Support Opportunities: Research Grants - State Research Institutes Modernization Program