Multi-User Equipment approved in grant 2017/50333-7: MALDI-TOF

• Grant number: 18/21192-9
• Support type: Multi-user Equipment Program
• Duration: December 01, 2018 - November 30, 2025
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal researcher: José Eduardo Tolezano
• Grantee: José Eduardo Tolezano
• Home Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 17/50333-7 - Institutional research development plan of the Instituto Adolfo Lutz (PDIp), AP.PDIP
• EMU web page: http://www.ial.sp.gov.br/ial/serviços/equipamentos-multiusuarios
• Type of equipment: Caracterização de Materiais - Espectrometria - De massas
• Manufacturer: Bruker
• Model: MALDI TOF-Biotyper 3.0

Abstract

The Institutional Research Development Plans of the State Research Institutes in the State of São Paulo was designed to improve and to transform the research and diagnostics in Public Health in the next few years. The PDIP was settled to protect, prevent and promote health and life's quality of the population. To achieve such aims, modernization of equipment was proposed, including the implementation of MALDI-TOF equipment as a multiuser platform. Mass spectrometry is a method that can be of great help in microbiological researcher and diagnosis. This technique is based on ionization and subsequent biomolecular detection. The Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) has been the most applied, is an ionization technique in which the ions formed have low internal energy, allowing the observation of ionized molecules with little or no fragmentation. The main advantage of MALDI-TOF MS is identification through bacterial protein profile analysis rather than physical, biochemical and metabolic differentiation. Compared with conventional phenotypic methods or PCR, MALDI-TOF MS has been increasingly applied in the field of diagnostic microbiology and has begun to replace biochemical methods. It is a lower cost method when compared to some molecular and immunological techniques, and does not require specialized labor. In addition, this methodology allows quick answers to be obtained, surpassing other conventional methodologies, providing information that allows the immediate decision making by health professionals, regarding control and prevention measures, detection of source of infection, transmission routes and zoonotic control. The applications of sequencing can be broadly amplified, allowing the utilization of the service by users of Bacteriology, Food Center, Collections of cultures, Parasitology and Mycology, Immunology Divisions, and to the 13 Centers of Regional Laboratories, for diagnosis and research. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YAMADA, AMANDA YAEKO; SANTOS, ALESSANDRO MARQUES DOS; SOUZA, ANDREIA RODRIGUES DE; CAMPOS, KAROLINE RODRIGUES; SACCHI, CLAUDIO TAVARES; TIBA-CASAS, MONIQUE RIBEIRO; CAMARGO, CARLOS HENRIQUE. A clinical Pseudomonas chlororaphis isolate harboring a new blaVIM-2 borne integron, In2088, isolated from bloodstream infection in a newborn patient. INFECTION GENETICS AND EVOLUTION, v. 96, DEC 2021. Web of Science Citations: 0.