Antimicrobial resistance genotypes and phenotypes of Campylobacter coli isolated from different sources over a 16-year period in Brazil

Author(s)

Gomes, Carolina Nogueira ; Campioni, Fabio ; Barker, Dillon Oliver Reese ; Che, Emily Victoria ; Duque, Sheila da Silva ; Taboada, Eduardo Napoleon ; Falcao, Juliana Pfrimer

• Document type: Journal article
• Source: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 33, p. 5-pg., 2023-06-01.

Abstract

Objectives: This study aimed to identify antimicrobial resistance genotypes in 63 Campylobacter coli strains isolated from humans (12), animals (21), the environment (20), and food (10) in Brazil using whole genome sequencing (WGS) tools, comparing them with results obtained by antimicrobial susceptibility testing (AST) against some important antimicrobials in clinical use.Methods: Phenotypic resistance profiles were determined by minimal inhibitory concentrations and the disk diffusion technique. The prediction of the resistance genes was performed using ABRicate v.0.8 and the Resistance Gene Identifier software of the CARD. Results: The percentage of C. coli strains phenotypically resistant to antimicrobials were: ampicillin, 44.4%; doxycycline, 20.6%; tetracycline, 20.6%; ciprofloxacin, 12.7%; nalidixic acid, 12.7%; streptomycin, 6.3%; erythromycin, 4.8%; and gentamicin, 1.6%. The genes bla OXA-605 / bla OXA-61, tet (O), cme B, aadE -Cc, aph (3 ') - IIIa, sat 4 and aad 9 were detected in 54%, 22.2%, 9.5%,6.3%, 1.6%, 1.6%, and 1.6% strains, respectively. Mutations T86I in the QRDR region of gyr A were detected in 8 (12.7%) strains. The agreement between AST and WGS was 100%, 92.9%, 82.4%, and 80% for quinolones, tetracycline, & beta;-lactam, and aminoglycoside classes, respectively.Conclusions: The rates of C. coli strains resistant to & beta;- lactams and quinolones may represent a public health concern. The partial agreement between AST and WGS shows that improvement in antibiotic resistance databases may be required to minimize this discrepancy observed in some antimicrobial classes and to become an acceptable tool to both clinical microbiologists and regulatory agencies. & COPY; 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) (AU)

• FAPESP's process: 22/07013-0 - Genome analyses, transcriptome, mutants' construction and phenotypic characterization of Campylobacter coli strains isolated from different sources during 25 years in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/26043-1 - Whole genome sequencing and comparative genomics of Campylobacter coli strains isolated from different sources over a 16 year-period in Brazil
• Grantee: Carolina Nogueira Gomes
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/24716-3 - Sequencing of the Genome, transcriptoma and phenotypic analysis of Campylobacter coli strains isolated from different sources in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/19338-8 - Genomic, transcriptomic and phenotypic characterization of Campylobacter jejuni strains isolated from diverse source during 20 years in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/23408-0 - Phenotypic analysis, genome and transcriptome sequencing of Campylobacter coli strains isolated from different sources during 16 years in Brazil
• Grantee: Carolina Nogueira Gomes
• Support Opportunities: Scholarships in Brazil - Doctorate