Toxicity and mutagenicity studies of 6PPD-quinone in a marine invertebrate species and bacteria

Author(s)

Botelho, Marina Tenorio ; Militao, Gabriely Groto ; Brinkmann, Markus ; Umbuzeiro, Gisela de Aragao

• Document type: Journal article
• Source: Environmental and Molecular Mutagenesis; v. 64, n. 6, p. 7-pg., 2023-07-17.

Abstract

N-(1,3-dimethylbutyl)-N & PRIME;-phenyl-p-phenylenediamine-quinone (6PPD-quinone), an oxidation product of the tire additive, 6PPD, has been associated with high mortality of salmonids (0.1 & mu;g/L). The objective of this study was to determine the acute toxicity using neonates and mutagenicity (micronuclei in hemolymph of exposed adults) of 6PPD-quinone in the marine amphipod Parhyale hawaiensis. Also, we studied its mutagenicity in the Salmonella/microsome assay using five strains of Salmonella with and without metabolic system (rat liver S9, 5%). 6PPD-quinone did not present acute toxicity to P. hawaiensis from 31.25 to 500 & mu;g/L. Micronuclei frequency increased after 96 h-exposure to 6PPD-quinone (250 and 500 & mu;g/L) when compared to the negative control. 6PPD-quinone also showed a weak mutagenic effect for TA100 only in the presence of S9. We conclude that 6PPD-quinone is mutagenic to P. hawaiensis and weakly mutagenic to bacteria. Our work provides information for future risk assessment of the presence of 6PPD-quinone in the aquatic environment. (AU)

• FAPESP's process: 20/04628-8 - ToxBioColour: toxicology of bio-based colourants for a sustainable environment
• Grantee: Gisela de Aragão Umbuzeiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/04482-9 - Developing the marine amphipod Parhyale hawaiensis as a new platform for germ cells mutagenicity assessment
• Grantee: Marina Tenório Botelho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral