Identifying markers volatiles in Brazilian virgin oil by multiple headspace solid-phase microextraction, and chemometrics tools

Author(s)

Lima, Adriano Freitas ; Oliveira, Wellington da Silva ; Garcia, Aline de Oliveira ; Vicente, Eduardo ; Godoy, Helena Teixeira

• Document type: Journal article
• Source: Food Research International; v. 167, p. 14-pg., 2023-03-22.

Abstract

A protocol was optimized to determine the volatile profile from monovarietal virgin olive oil (VOO) by multiple headspace solid-phase microextraction (MHS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. For this, a Plackett-Burman (PB) and central composite rotational designs (CCRD) were used to define the best condition of extraction. Moreover, fatty acids profile and principal component analysis (PCA) was used to identify markers among the cultivars. The amount of 0.1 g of sample was enough to express the volatile composition of the olive oils by MHS-SPME. Volatile compounds [nonanal, (Z)-3-Hexen-1-ol, (Z)-3Hexenyl Acetate, Hexyl Acetate, 3-Methylbutyl Acetate, (E)-2-Hexen-1-ol, (E)-2-Hexenyl Acetate] and fatty acids [C17:1, C18, C18:1, C18:2] were those reported such as the markers in the varieties of olive oils. The PCA analysis allowed the classification of the most representative volatiles and fatty acids for each cultivar. Through two principal components was possible to obtain 81.9% of explanation of the variance of the compounds. The compounds were quantified using a validated method. The MHS-SPME combined with multivariate analysis showed a promising tool to identify markers and for the discrimination of olive oil varieties. (AU)

• FAPESP's process: 20/02728-5 - Evaluation of the overall quality of coffee beverage based on olfatometry and GC-MS/MS
• Grantee: Wellington da Silva Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/00121-9 - Multi-User Equipment approved in grant 2017/50349-0: gas chromatograph coupled to mass spectrometer triple quadrupole - GC-MS/MS with an olfactometer detector as accessory
• Grantee: Eloísa Elena Corrêa Garcia
• Support Opportunities: Multi-user Equipment Program