Effect of experimental toothpaste containing hydroxyapatite nanoparticles and propolis, with and without fluoride, on the microcosm biofilm and enamel demineralization

Author(s)

Ribeiro, Caroline Fernanda da Silva ; Dionizio, Aline ; Araujo, Tamara Teodoro ; Rodrigues, Chelsea Maria Vilas Boas Feitosa ; Mattos, Amanda Costa ; Otaviano, Leticia Galli ; Grizzo, Larissa Tercilia ; Magalhaes, Ana Carolina ; Buzalaf, Marilia Afonso Rabelo

• Document type: Journal article
• Source: BIOFOULING; v. 39, n. 3, p. 10-pg., 2023-05-26.

Abstract

This study evaluated the antimicrobial and anticaries effects of toothpaste containing hydroxyapatite nanoparticles (nanoHAP - 5 or 10%), xylitol (2 or 3%) and propolis (1 or 2%), associated or not with 1500 ppm fluoride (F). An in vitro model was used with microcosm biofilm produced from a pool of human saliva and McBain saliva (1:50) in the first 8 h of culture on 162 bovine enamel specimens. At the end of the experimental period, analyses of metabolic activity, colony forming units (CFU) and transverse microradiography (TMR) were performed. This study showed a possible decrease in demineralization and increase in remineralization by the commercial toothpaste (1500 ppm F) and for the experimental toothpaste containing the highest concentration of all agents, combined with F. In addition, a reduction in antimicrobial activity possibly caused by propolis and xylitol, mainly in relation to cariogenic bacteria, was observed. (AU)

• FAPESP's process: 19/03866-5 - Evaluation of the efficiency of experimental dentifrices with hydroxypatian nanoparticles with or without fluoride, in the prevention of dental care
• Grantee: Caroline Fernanda da Silva Ribeiro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation