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Evaluation of the efficiency of experimental dentifrices with hydroxypatian nanoparticles with or without fluoride, in the prevention of dental care

Grant number: 19/03866-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2019
Effective date (End): June 30, 2022
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Caroline Fernanda da Silva Ribeiro
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Dentifrices are probably the most used products in dentistry. New biofunctional products, such as those containing hydroxyapatite or nanoparticles of hydroxyapatite (nanoHAP), are being developed with the aim of interfering positively in the development and progression of dental caries. The objective of this study was to evaluate the efficacy of new dentifrices with nanoHAP active substances in different concentrations and propolis, associated or not to fluoride, in non cavitated caries lesions (white spot lesions) in order to reduce demineralization and accelerate the remineralization of bovine enamel. For this purpose, we will carry out an in vitro study using blocks of bovine enamel (4x4 mm, n = 12 per group), which will initially be submitted to the formation of initial lesions of caries. After the formation of the lesions, the blocks will be subdivided into 7 groups, according to the dentifrice used, namely: Group 1: placebo dentifrice (negative control); Group 2: Crest dentifrice (P & G, positive control); Group 3: Premium dentifrice: 10% nanoHAP, 5% propolis, 3% xylitol; Group 4: Premium + fluoride dentifrice: 10% nanoHAP, 5% propolis, 3% xylitol, 1500 ppm fluoride (as MFP); Group 5: Combination dentifrice: 5% nanoHAP, 2% propolis, 1% xylitol; Group 6: dentifrice Combate + fluoride: 5% nanoHAP, 2% propolis, 1% xylitol, 1500 ppm fluoride (as MFP) and Group 7: fluoride and propolis dentifrice: 2% propolis, 1500 ppm fluorine (as MFP). The blocks will be submitted to treatment with the dentifrices during 7 days, interspersed with pH cations (RE> DES). The superficial hardness will be evaluated with a microdurometer during three moments of the experiment: healthy tooth (before caries lesion), post caries lesion and post treatment with the dentifrices, to calculate the percentage of surface hardness recovery. The data will be analyzed in relation to normality and homogeneity, to seal the appropriate statistical test (p <0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO, CAROLINE FERNANDA DA SILVA; DIONIZIO, ALINE; ARAUJO, TAMARA TEODORO; RODRIGUES, CHELSEA MARIA VILAS BOAS FEITOSA; MATTOS, AMANDA COSTA; OTAVIANO, LETICIA GALLI; GRIZZO, LARISSA TERCILIA; MAGALHAES, ANA CAROLINA; BUZALAF, MARILIA AFONSO RABELO. Effect of experimental toothpaste containing hydroxyapatite nanoparticles and propolis, with and without fluoride, on the microcosm biofilm and enamel demineralization. BIOFOULING, v. 39, n. 3, p. 10-pg., . (19/03866-5)

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