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Evaluation OF “The effect OF calcium rinse followed by use OF fluoridated dentifrice ón erosion ánd abrasion OF bovine dentin ín vitro

Grant number: 12/14667-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Even Akemi Taira
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


This project will evaluate the effect of a mouthwash with calcium before fluoride toothpaste on erosion associated to abrasion of bovine dentin in vitro. During 5 days, bovine dentin blocks (n = 20) will be submitted the erosion + abrasion. The study will include the following treatments: rinsing with calcium and fluoride dentifrice (BCa + DF), rinse with calcium and placebo dentifrice (BCa + DP), rinsed with deionized water and using fluoridated dentifrice (BP + DF) and rinse with deionized water and dentifrice placebo (BP + DP). The erosion will be conducted 4 times per day for cola type refrigerant (90 s each), for 5 days. For abrasion, the blocks will be brushed, using electric toothbrush (Oral B-Pro Health Power) and the dentifrice slurry to be used for each group (3 g: 10 mL deionized water) for 15 s, 2x/day. Between the erosion and abrasion, each block will be immersed in 10 mL of deionized water or solution of 150 mM calcium lactate for 1 min, with stirring, 2x/day. The response variable will be the analysis by profilometry (µm). The data will be checked for normality and homogeneity (Bartlett's test / Kolmogorov and Smirnov), and will apply the most appropriate statistical test results (p <0.05).Keywords: calcium, dental erosion, tooth abrasion, dentin, hydroxyapatite, remineralization, fluoride toothpaste.