Effect of TiF4 on hydroxiapatite and enamel subjected to cariogenic and erosive processes: in vitro and in situ studies

• Grant number: 12/20698-0
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2013
• End date: September 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Ana Carolina Magalhães
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Marília Afonso Rabelo Buzalaf

Abstract

The aim of this study is to evaluate the interaction and effect of TiF4, included in different formulations (varnish, dentifrice and mouth rinse), on enamel subjected to cariogenic and erosive/abrasive challenges. This project will be divided into three parts. The first study involves doctoral/PQ/IC projects, in which it will be assessed in vitro (A) the quantity of fluoride release into the water from TiF4 and NaF varnishes with different F concentrations (0.95-2.45% F-) along 12h; (B) the type of compounds formed with synthetic hydroxyapatite after the application of TiF4 e NaF solutions using infra-red spectroscopy; (C) the presence of elements on sound and demineralized bovine and human enamel treated with the fluoride varnishes using scanning electron microscopy and microanalysis; (D) the formation of CaF2 on sound and demineralized bovine and human enamel treated with the fluoride varnishes using the KOH-soluble biopsy; (E) the incorporation of fluoride (FAP) using the acidic ans abrasive biopsy and (F) the loss or gain of mineral as well as the lesion depht alteration after the treatments with TiF4 and NaF followed by de-remineralization of bovine or human enamel with artificial carious lesion, changing the remineralization conditions in situ. The change in mineral content will be analysed using transverse microradiography. The second study involves IC project, in which the effect of TiF4, NaF and SnF2 dentifrices (1500 ppm F) against bovine enamel erosion and abrasion will be compared. The quantity of total, soluble and ionic F, pH and REA values of the experimental dentifrices will be checked. A pH-cycling model will be applied, in which the enamel samples will be exposed to the erosive agent (4x90s/day) and abrasive toothbrushing using the experimental dentifrices (2x15s/day, toothbrushing machine), with intervals of exposition to artificial saliva, for 7 days. Enamel loss will be measured using contact profilometry. Finally, the third study will also involves IC, in which the preventive effect of TiF4, NaF and SnF2 mouth rinses (500 ppm F) against bovine enamel erosion will be compared. For this study, the same pH cycling model from the 2nd study will be applied, however, in this case there will be no abrasion. Instead, the samples will be exposed to the fluoride solutions 2x1 min/day. Enamel loss will be measured using contact profilometry. The data will be submitted to the statistical analysis acording to the experimental model (p<0.05). (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COMAR, L. P.; SOUZA, B. M.; MARTINS, J.; SANTOS, M. G.; BUZALAF, M. A. R.; MAGALHAES, A. C.. Response of carious enamel to TiF4 varnish treatment under diverse cariogenic activities in situ. Journal of Dentistry, v. 63, p. 81-84, AUG 2017. (11/11263-7, 12/00662-0, 12/20698-0)

MAGALHAES, ANA CAROLINA; DOS SANTOS, MILENA GARGARO; COMAR, LIVIA PICCHI; RABELO BUZALAF, MARILIA AFONSO; GANSS, CAROLINA; SCHLUETER, NADINE. Effect of a Single Application of TiF4 Varnish versus Daily Use of a Low-Concentrated TiF4/NaF Solution on Tooth Erosion Prevention in vitro. Caries Research, v. 50, n. 5, p. 462-470, 2016. (12/22642-1, 12/20698-0, 14/23472-8)

LIVIA PICCHI COMAR; BEATRIZ MARTINES DE SOUZA; LARISSA TERCILIA GRIZZO; MARÍLIA AFONSO RABELO BUZALAF; ANA CAROLINA MAGALHÃES. Evaluation of fluoride release from experimental TiF4 and NaF varnishes in vitro. Journal of Applied Oral Science, v. 22, n. 2, p. 138-143, 2014-04-00. (10/07001-4, 11/11263-7, 12/20698-0)

DE SOUZA, BEATRIZ MARTINES; VERTUAN, MARIELE; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. The Impact of the Demineralized Organic Matrix on the Effect of TiF4 Varnish on the Progression of Dentin Erosive Loss. Caries Research, v. 51, n. 3, p. 264-270, 2017. (13/20372-0, 12/20698-0, 13/26237-7)

FREIRE DE CASTILHO, ALINE ROGERIA; ARANDA SALOMAO, PRISCILA MARIA; RABELO BUZALAF, MARLIA AFONSO; MAGALHAES, ANA CAROLINA. Protective effect of experimental mouthrinses containing NaF and TiF4 on dentin erosive loss in vitro. Journal of Applied Oral Science, v. 23, n. 5, p. 486-490, SEP-OCT 2015. (12/20698-0)

COMAR, LIVIA P.; SOUZA, BEATRIZ M.; AL-AHJ, LUANA P.; MARTINS, JESSICA; GRIZZO, LARISSA T.; PIASENTIM, ISABELLE S.; RIOS, DANIELA; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Mechanism of Action of TiF4 on Dental Enamel Surface: SEM/EDX, KOH-Soluble F, and X-Ray Diffraction Analysis. Caries Research, v. 51, n. 6, p. 554-567, 2017. (12/00662-0, 13/20372-0, 12/20698-0, 11/11263-7, 10/07001-4)