| Full text | |
| Author(s): Show less - |
Passos, Larissa Souza
;
Jacinavicius, Fernanda Rios
;
Geraldes, Vanessa
;
de Freitas, Paloma Nathane Nunes
;
Da Silva, Gabriela Helena
;
de Almeida, Eryka Costa
;
Alves, Angelica Priscila do Carmo
;
Orlando, Tamira Maria
;
Cerozi, Brunno da Silva
;
Martinez, Diego Stefani Teodoro
;
Pinto, Ernani
Total Authors: 11
|
| Document type: | Journal article |
| Source: | Chemosphere; v. 332, p. 12-pg., 2023-05-12. |
| Abstract | |
Anthropogenic activity has dramatically deteriorated aquatic ecosystems in recent years. Such environmental alterations could change the primary producers' composition, exacerbating the proliferation of harmful micro-organisms such as cyanobacteria. Cyanobacteria can produce several secondary metabolites, including guani-toxin, a potent neurotoxin and the only naturally occurring anticholinesterase organophosphate ever reported in the literature. Therefore, this study investigated the acute toxicity of guanitoxin-producing cyanobacteria Sphaerospermopsis torques-reginae (ITEP-024 strain) aqueous and 50% methanolic extracts in zebrafish (Danio rerio) hepatocytes (ZF-L cell line), zebrafish embryos (fish embryo toxicity -FET) and specimens of the micro -crustacean Daphnia similis. For this, hepatocytes were exposed to 1-500 mg/L of the ITEP-024 extracts for 24 h, the embryos to 31.25-500 mg/L for 96 h, and D. similis to 10-3000 mg/L for 48 h. Non-target metabolomics was also performed to analyze secondary metabolites produced by the ITEP-024 using LC-MS/MS. Metabolomics indicated the guanitoxin presence just in the aqueous extract of the ITEP-024 and the presence of the cyano-peptides namalides, spumigins, and anabaenopeptins in the methanolic extract. The aqueous extract decreased the viability of zebrafish hepatocytes (EC(I)50(24h) = 366.46 mg/L), and the methanolic extract was not toxic. FET showed that the aqueous extract (LC50(96) = 353.55 mg/L) was more toxic than the methanolic extract (LC50(96) = 617.91 mg/L). However, the methanolic extract had more sublethal effects, such as abdominal and cardiac (cardiotoxicity) edema and deformation (spinal curvature of the larvae). Both extracts immobilized daphnids at the highest concentration analyzed. However, the aqueous extract was nine times more lethal (EC(I)50(48h) = 108.2 mg/L) than the methanolic extract (EC(I)50(48h) = 980.65 mg/L). Our results showed an imminent bio-logical risk for aquatic fauna living in an ecosystem surrounded by ITEP-024 metabolites. Our findings thus highlight the urgency of understanding the effects of guanitoxin and cyanopeptides in aquatic animals. (AU) | |
| FAPESP's process: | 20/00109-6 - Cyanopeptide toxicity assessment in fish for human consumption from artificial ponds |
| Grantee: | Larissa Souza Passos |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/07914-8 - FoRC - Food Research Center |
| Grantee: | Bernadette Dora Gombossy de Melo Franco |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 21/00149-0 - Treatment and selective fractionation of cyanobacteria in blooms for the production of value-added supplies |
| Grantee: | Ernani Pinto Junior |
| Support Opportunities: | Regular Research Grants |