Citrus flavanone metabolites significantly modulate global proteomic profile in pancreatic beta-cells under high-glucose-induced metabolic stress

Author(s)

Fraga, Layanne Nascimento ; Milenkovic, Dragan ; Anacleto, Sara Lima ; Salemi, Michelle ; Lajolo, Franco Maria ; Hassimotto, Neuza Mariko Aymoto

• Document type: Journal article
• Source: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1871, n. 3, p. 21-pg., 2023-02-02.

Abstract

Hesperidin and narirutin are the major citrus flavanones. Several studies have associated these compounds with pancreatic beta-cell survival through their capacity to reduce oxidative stress, inflammation, and inhibit apoptosis. However, the molecular mechanisms of action of flavanones in pancreatic beta-cells under high-glycemic stress is still largely unknown. Therefore, this study aimed to decipher molecular mechanisms of flavanone metabolites in pancreatic beta-cells treated with high glucose concentration using untargeted shotgun proteomics. We identified 569 proteins differentially expressed in cells exposed to hesperetin 7-glucuronide (H7G) and 265 in cells exposed to 3-(4'-hydroxyphenyl) propanoic acid (PA). Comparison of global proteomic profiles suggest that these metabolites could counteract changes in protein expression induced by high glucose stress. The bioinformatic analyses suggested that H7G and PA modulated the expression of proteins involved in cell adhesion, cell signaling, metabolism, inflammation, and protein processing in endoplasmic reticulum (ER) pathways. Taken together, this study suggests that H7G and PA can modulate the expression of proteins that may prevent dysfunction of pancreatic beta-cells under stress induced by high glucose. (AU)

• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 18/26584-2 - Effect of citrus flavanones in Min6 cells under oxidative stress: Proteomic approach
• Grantee: Sara Lima Anacleto
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 18/03965-0 - Citrus Flavanones- mechanisms of protection of pancreatic-beta cells under oxidative stress
• Grantee: Sara Lima Anacleto
• Support Opportunities: Scholarships in Brazil - Master