| Full text | |
| Author(s): Show less - |
Sano, Elder
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Fontana, Herrison
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Esposito, Fernanda
;
Cardoso, Brenda
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Fuga, Bruna
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Costa, Gladyston C. V.
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Bosqueiro, Tatiana C. M.
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Sinhorini, Juliana A.
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Orico, Lilian D.
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de Masi, Eduardo
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Aires, Caroline C.
;
Lincopan, Nilton
Total Authors: 12
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| Document type: | Journal article |
| Source: | JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 33, p. 4-pg., 2023-05-14. |
| Abstract | |
Objectives: The aim of this study was to perform a genomic investigation of a multiple fluoroquinolone-resistant Leclercia adecarboxylata strain isolated from a synanthropic pigeon in Sao Paulo, Brazil.Methods: Whole-genome sequencing was performed using an Illumina platform, and in silico deep analy-ses of the resistome were performed. Comparative phylogenomics was conducted using a global collection of publicly available genomes of L. adecarboxylata strains isolated from human and animal hosts.Results: L. adecarboxylata strain P62P1 displayed resistance to human (norfloxacin, ofloxacin, ciprofloxacin, and levofloxacin) and veterinary (enrofloxacin) fluoroquinolones. This multiple quinolone-resistant profile was associated with mutations in the gyrA (S83I) and parC (S80I) genes and the presence of the qnrS gene within an ISKpn19-orf-qnrS1-4IS3-blaLAP-2 module, previously identified in L. adecar-boxylata strains isolated from pig feed and faeces in China. Genes associated with arsenic, silver, copper, and mercury resistance were also predicted. Phylogenomic analysis revealed clustering (378-496 single nucleotide polymorphism differences) with two L. adecarboxylata strains isolated from human and fish sources in China and Portugal, respectively.Conclusions: L. adecarboxylata is a Gram-negative bacterium of the Enterobacterales order and is con-sidered an emergent opportunistic pathogen. Since L. adecarboxylata has adapted to human and animal hosts, genomic surveillance is highly recommended, in order to identify the emergence and spread of re-sistant lineages and high-risk clones. In this regard, this study provides genomic data that can help clarify the role of synanthropic animals in the dissemination of clinically relevant L. adecarboxylata within a One Health context.(c) 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) (AU) | |
| FAPESP's process: | 20/08224-9 - OneBR (One Health Brazilian Resistance): integrated genomic database for surveillance, diagnosis, management and treatment of antimicrobial resistance in the human-animal-environment interface, in Brazil |
| Grantee: | Nilton Erbet Lincopan Huenuman |
| Support Opportunities: | Regular Research Grants |