Iron zero valent nanoparticles applied in the biorefinery concept: Functional genes in continuous bioreactor fermenting vinasse

Author(s)

Moura, Alana G. L. ; Delforno, Tiago P. ; Rabelo, Camila A. B. S. ; Silva, Edson L. ; Varesche, Maria B. A.

• Document type: Journal article
• Source: BIOMASS & BIOENERGY; v. 174, p. 14-pg., 2023-05-30.

Abstract

Increased production of biogas and volatile acids was evaluated by the addition of Fe0 Nanoparticles (NP) on Anaerobic Fluidized Bed Reactor (AFBR-NP) with expanded clay as support material (45.1 mg Fe0 g-1 expanded clay), fermenting vinasse, and operated at 37 & DEG;C and 3h Hydraulic Retention Time (HRT). Higher H2 percentage in the biogas composition was observed in AFBR-NP (50-65%) when compared to AFBR without NP, AFBRControl (24-43%). A 1.2-fold higher valeric acid production was also observed as a result of NPs supplementation. Genes related to the first steps of vinasse anaerobic degradation in AFBR-NP (beta-glucosidase, glucokinase and pyruvate-ferredoxin oxidoreductase) and the genes related to volatile acids production (L- and Dlactate dehydrogenase, 3-hydroxybutyryl-CoA dehydrogenase, acetate kinase, pyruvate kinase and fumarate reductase flavoprotein) were observed by metagenome sequencing. A 4.2-fold higher relative abundance of Secundilactobacillus genus in AFBR-NP was evidenced. The genetic and microbial community differences observed with the addition of NP resulted in improved biorefinery products of anaerobic digestion. (AU)

• FAPESP's process: 17/23008-8 - Assessment of Iron and Nickel nanoparticles influence on batch and continuous reactors fermentative hydrogen production
• Grantee: Alana Gandra Lima de Moura
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/26470-7 - Influence of sugarcane bagasse saccharification by laccase for fermentative production of biofuels
• Grantee: Camila Abreu Borges da Silva Rabelo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral