The feruloyl esterase from Thermobacillus xylanilyticus shows broad specificity for processing pre-biotic feruloylated xylooligosaccharides at high temperatures

Author(s)

Garbelotti, Carolina V. ; Bulmer, Gregory S. ; Ward, Richard J. ; van Munster, Jolanda M.

• Document type: Journal article
• Source: Food Chemistry; v. 405, p. 8-pg., 2023-03-30.

Abstract

Ferulic acid has antioxidant properties of interest to the food industry and can be released from natural plant fibres using feruloyl esterases. Esterases active at high temperatures are highly desirable but currently underrepresented. Here we report the biochemical characterization of the feruloyl esterase from Thermobacillus xylanilyticus. Specific activity of recombinant Tx-Est1 with ethyl ferulate was 29.2 & PLUSMN; 2.9 U mg-1, with a catalytic efficiency (Kcat/Km) of 393.7 & PLUSMN; 9.8 s-1mM- 1. The temperature and pH optima were 60 degrees C and 7.5, whereby TxEst1 retains 70% activity after 25 h at 40 degrees C. MALDI-TOF MS revealed Tx-ESTI released ferulic acid from xylooligosaccharides with DP4-DP13, and from DP6-8 containing two ferulic acid groups. HPLC demonstrated ferulic acid release from destarched wheat bran was strongly potentiated by co-incubation with xylanase. These properties, especially the high activity at elevated temperatures, suggest Tx-Est1 can be employed for production of high-value compounds from agricultural waste or during plant polysaccharide saccharification. (AU)

• FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/14030-2 - Understanding plant cell wall architecture to improve enzymatic hydrolysis of lignocellulose
• Grantee: Richard John Ward
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/25664-2 - Expression and purification of recombinants xylanolitics enzymes and study of their activities against sugar cane extracted arabinoxylans
• Grantee: Carolina Victal Garbelotti
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)