Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar rats

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Favero, Aline Garnevi ; da Silva, Karina Nascimento ; Ribeiro, Willian Rodrigues ; Ferreira, Caroline Marcantonio ; Sartorelli, Patricia ; Cardili, Leonardo ; Sinigaglia, Rita de Cassia ; Pereira, Joice Naiara Bertaglia ; da Silva, Marcelo Aparecido ; Vilegas, Wagner ; Silva, Marcelo Jose Dias ; Paiotti, Ana Paula Ribeiro

• Document type: Journal article
• Source: BIOMEDICA; v. 43, n. 2, p. 33-pg., 2023-06-01.

Abstract

Introduction: Anti-inflammatories, immunosuppressants, and immunobiological they commonly used as the treatment of inflammatory bowel disease. However, some patients do not present an adequate response or lose effective response during the treatment. In a recent study, we found a potential anti-inflammatory effect of the hydroalcoholic extract of Mimosa caesalpiniifolia on trinitrobenzene sulfonic acid -induced colitis in Wistar rats.Objective: To evaluate the effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier using dextran sulfate sodium-induced colitis model. Materials and methods: Leaf extracts were prepared in 70% ethanol and dried with a Buchi B19 Mini-spray dryer using 20% aerosil & REG;. Thirty-two male Wistar rats were randomized into four groups: basal control, untreated colitis, pre-formulation control (125mg/kg/d), and colitis treated with pre-formulation (125mg/kg/d). Clinical activity index was recorded daily and all rats were euthanized on the 9th day. Colon fragments were fixed and processed for histological and ultrastructural analyses. Stool samples were collected and processed for analysis of the short-chain fatty acid. Results: Treatment with the pre-formulation decreased the clinical activity, inflammatory infiltrate, and the ulcers. Pre-formulation did not repair the epithelial barrier and there were'nt no significant differences in the goblet cells index. There was a significant difference in butyrate levels in the rats treated with the pre-formulation.Conclusions: The pre-formulation minimized the clinical symptoms of colitis and intestinal inflammation, but did not minimize damage to the intestinal barrier. (AU)

• FAPESP's process: 15/21479-8 - Standardization of plant extracts of Mimosa spp. development phytomedicines
• Grantee: Marcelo José Dias Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral