Effect of maternal supplementation with retinoic acid during breastfeeding on the immune response of mice offspring

• Grant number: 17/18199-9
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2019 - January 31, 2021
• Field of knowledge: Biological Sciences - Immunology
• Principal Investigator: Maria Notomi Sato
• Grantee: Maria Notomi Sato
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Vitamin A through its active metabolite retinoic acid is able to modulate the immune response and maintain mucosal homeostasis, especially in immunological sites such as the GutAssociated Lymphoid Tissue. The neonatal period is characterized by relative immunological immaturity and increased susceptibility to infections. In addition, the neonate is born with low concentrations of vitamin A and the neonate receives the vitamin through breastfeeding. Thus, we propose to evaluate the effect of maternal supplementation with retinoic acid during the breastfeeding period on the immune response and on the mucosa and microbiota intestinal of the offspring mice. To this, we will evaluate the presence of T cells in the spleen and mesenteric lymph nodes with migratory potential for the intestine, and the presence of regulatory and effector T cells and innate lymphoid cells in the small intestine of the offspring. The profile of pro- and anti-inflammatory cytokines, serum and fecal IgA antibodies and intestinal microbiota composition of the offspring will also be evaluated. In addition, the effect of maternal supplementation will be assessed in the intestinal inflammatory response by dextran sulfate sodium-induced colitis. The milk of retinoic acidsupplemented mothers will be evaluated for the presence of IgA and IgG antibodies and pro- and anti-inflammatory cytokines, for a better understanding of the impact of maternal supplementation with retinoic acid in this compartment. Using a system that the neonates will be transferred to nursed by another mother, the migration of maternal milk cells to mesenteric lymph nodes and Peyer's patches from offspring and the effector or regulatory functional capacity of these cells will be evaluated. Assessment of the effect of maternal and retinoic acid supplementation during breastfeeding will provide an understanding of the benefits and/or harms of a diet rich in this compound in the development of the offspring immune system and have a potential implication in human health. Keywords: maternal-fetal interaction, retinoic acid, maternal milk, intestinal mucosa, intestinal microbiota, colitis and neonatal immunity. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALBERCA, RICARDO WESLEY; TEIXEIRA, FRANCIANE MOURADIAN EMIDIO; BESERRA, DANIELLE ROSA; DE OLIVEIRA, EMILY ARAUJO; ANDRADE, MILENA MARY DE SOUZA; PIETROBON, ANNA JULIA; SATO, MARIA NOTOMI. Perspective: The Potential Effects of Naringenin in COVID-19. FRONTIERS IN IMMUNOLOGY, v. 11, SEP 25 2020. (19/02679-7, 17/18199-9, 18/18230-6)

TEIXEIRA, FRANCIANE MOURADIAN EMIDIO; PIETROBON, ANNA JULIA; OLIVEIRA, LUANA DE MENDONCA; OLIVEIRA, LUANDA MARA DA SILVA; SATO, MARIA NOTOMI. Maternal-Fetal Interplay in Zika Virus Infection and Adverse Perinatal Outcomes. FRONTIERS IN IMMUNOLOGY, v. 11, FEB 14 2020. (17/18199-9, 18/18230-6)

GOZZI-SILVA, SARAH CRISTINA; BENARD, GIL; ALBERCA, RICARDO WESLEY; YENDO, TATIANA MINA; EMIDIO TEIXEIRA, FRANCIANE MOURADIAN; OLIVEIRA, LUANA DE MENDONCA; BESERRA, DANIELLE ROSA; PIETROBON, ANNA JULIA; DE OLIVEIRA, EMILY ARAUJO; CALVIELLI CASTELO BRANCO, ANNA CLAUDIA; et al. SARS-CoV-2 Infection and CMV Dissemination in Transplant Recipients as a Treatment for Chagas Cardiomyopathy: A Case Report. TROPICAL MEDICINE AND INFECTIOUS DISEASE, v. 6, n. 1, MAR 2021. (18/18230-6, 17/18199-9, 19/02679-7, 19/26928-6, 19/22448-0)

ALBERCA, RICARDO WESLEY; SOLIS-CASTRO, ROSA LILIANA; SOLIS-CASTRO, MARIA EDITH; CARDOSO, FERNANDA; DUARTE, ALBERTO JOSE DA SILVA; OLIVEIRA, LUANA DE MENDONCA; PEREIRA, NATALLI ZANETE; GOZZI-SILVA, SARAH CRISTINA; DE OLIVEIRA, EMILY ARAUJO; AOKI, VALERIA; et al. Platelet-Based Biomarkers for Diagnosis and Prognosis in COVID-19 Patients. LIFE-BASEL, v. 11, n. 10, OCT 2021. (19/02679-7, 20/13148-0, 19/22448-0, 17/18199-9)

PIETROBON, ANNA JULIA; TEIXEIRA, FRANCIANE MOURADIAN EMIDIO; SATO, MARIA NOTOMI. I mmunosenescence and Inflammaging: Risk Factors of Severe COVID-19 in Older People. FRONTIERS IN IMMUNOLOGY, v. 11, OCT 27 2020. (17/18199-9, 18/18230-6)

ALBERCA, RICARDO WESLEY; YENDO, TATIANA; AOKI, VALERIA; SATO, MARIA NOTOMI. Asthmatic patients and COVID-19: Different disease course?. ALLERGY, v. 76, n. 3, SI, p. 963-965, MAR 2021. (19/02679-7, 17/18199-9)

ALBERCA, RICARDO WESLEY; LIMA, JULIA CATALDO; DE OLIVEIRA, EMILY ARAUJO; GOZZI-SILVA, SARAH CRISTINA; LEUZZI RAMOS, YASMIM ALEFE; DE SOUZA ANDRADE, MILENA MARY; BESERRA, DANIELLE ROSA; OLIVEIRA, LUANA DE MENDONCA; CALVIELLI CASTELO BRANCO, ANNA CLAUDIA; PIETROBON, ANNA JULIA; et al. COVID-19 Disease Course in Former Smokers, Smokers and COPD Patients. FRONTIERS IN PHYSIOLOGY, v. 11, JAN 27 2021. (19/02679-7, 17/18199-9)

ALBERCA, RICARDO WESLEY; DE SOUZA ANDRADE, MILENA MARY; CALVIELLI CASTELO BRANCO, ANNA CLAUDIA; PIETROBON, ANNA JULIA; PEREIRA, NATALLI ZANETE; FERNANDES, IARA GRIGOLETTO; OLIVEIRA, LUANA DE MENDONCA; EMIDIO TEIXEIRA, FRANCIANE MOURADIAN; BESERRA, DANIELLE ROSA; DE OLIVEIRA, EMILY ARAUJO; et al. Frequencies of CD33+CD11b+HLA-DR-CD14-CD66b+and CD33+CD11b+HLA-DR-CD14+CD66b-Cells in Peripheral Blood as Severity Immune Biomarkers in COVID-19. FRONTIERS IN MEDICINE, v. 7, OCT 14 2020. (19/02679-7, 17/18199-9, 19/26928-6, 18/18230-6, 19/22448-0)

ALBERCA, RICARDO WESLEY; RIGATO, PAULA ORDONHEZ; LEUZZI RAMOS, YASMIM ALEFE; EMIDIO TEIXEIRA, FRANCIANE MOURADIAN; CALVIELLI BRANCO, ANNA CLAUDIA; FERNANDES, IARA GRIGOLETTO; PIETROBON, ANNA JULIA; DA SILVA DUARTE, ALBERTO JOSE; AOKI, VALERIA; ORFALI, RAQUEL LEAO; et al. Clinical Characteristics and Survival Analysis in Frequent Alcohol Consumers With COVID-19. FRONTIERS IN NUTRITION, v. 8, JUN 2 2021. (17/18199-9)