Diversity of viruses and viroids in the rhizosphere of common bean cultivars differing in resistance to the fungal root pathogen Fusarium oxysporum

Author(s)

Braga, Lucas P. P. ; Tanentzap, Andrew J. ; Lee, Benjamin ; Tsai, Siu Mui ; Raaijmakers, Jos M. ; Mendes, Rodrigo ; Mendes, Lucas W.

• Document type: Journal article
• Source: APPLIED SOIL ECOLOGY; v. 190, p. 7-pg., 2023-06-22.

Abstract

The rhizosphere microbiome plays a key role in plant protection against soil-borne pathogens. Plant breeding for resistance against soil-borne pathogens can alter the rhizosphere microbiome. However, most studies have focused on bacterial and fungal communities, leaving the role of the virus and viroids unassessed. Here, we tested the influence of resistance breeding on the composition of rhizosphere viruses and viroids. By analyzing metatranscriptomes from the rhizosphere of common bean (Phaseolus vulgaris) cultivars with varying resistance to the soil-borne pathogen Fusarium oxysporum, we recovered sequences representing 78 and 23 novel populations of viruses and viroids, respectively. We compared the abundances of these infectious agents across the different cultivars and found that the Fusarium-resistant cultivar harbored >1.2 times more viroids and a more different composition of viroids and viruses than less resistant plants. Given their role in interfering with host metabolism and their potential influence on plant-fungi associations, our study suggests that changes in the rhizosphere infectome are an important consideration in breeding for resistance against soil-borne pathogens. (AU)

• FAPESP's process: 19/16043-7 - Rhizosphere microbiome of the drought tolerant common bean
• Grantee: Lucas William Mendes
• Support Opportunities: Research Program on Global Climate Change - Young Investigators
• FAPESP's process: 18/19247-0 - Unraveling the Role of Phages on the Evolution of Plant Beneficial and Pathogenic Rhizobacteria
• Grantee: Lucas Palma Perez Braga
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/03217-3 - Rhizosphere microbiome of common bean resistant to the soil pathogen Fusarium oxysporum
• Grantee: Lucas William Mendes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/00251-9 - Analysis of the microbiome associated to the common bean root system
• Grantee: Lucas William Mendes
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor