Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

Author(s)

de Oliveira, Analu Barros ; Ferrisse, Tulio Morandin ; Franca, Gabriela Gomes ; de Annunzio, Sarah Raquel ; Kopp, Willian ; Fontana, Carla Raquel ; Brighenti, Fernanda Lourencao

• Document type: Journal article
• Source: PATHOGENS; v. 12, n. 2, p. 19-pg., 2023-02-01.

Abstract

The synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log(10) CFU/mL (p < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (p < 0.0001). BGP-mediated aPDT delayed S. mutans and C. albicans regrowth after 24 h of treatment (p < 0.0001). Both BGP did not change the color of dental materials (p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p < 0.0001). BGP extracts may be used in aPDT against S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications. (AU)

• FAPESP's process: 19/08375-0 - Maximization of the anti-caries and antibiofilm effect of conventional substances: evaluation in a polymicrobial biofilm model
• Grantee: Fernanda Lourenção Brighenti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/23015-7 - Antimicrobial photodynamic therapy by continuous and switched mode irradiation against Enterococcus faecalis and Cutibacterium acnes
• Grantee: Carla Raquel Fontana
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18440-0 - Bioprospection of natural products in photodynamic therapy against microorganisms of medial and dental importance
• Grantee: Analú Barros de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 21/01191-0 - Tolerance induction and Candida albicans's biofilm characterization after antimicrobial photodymamic therapy application
• Grantee: Túlio Morandin Ferrisse
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/07110-0 - Potential of photodynamic therapy in modulating the inflammatory response and microbial virulence factors: evaluation in a model of three-dimensional co-cultures of gingival fibroblasts, Candida albicans and Staphylococcus aureus
• Grantee: Analú Barros de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate