Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels

Author(s)

Braga, Gabriela Pacheco de Almeida ; Caiaffa, Karina Sampaio ; Rabelo, Rafaela Laruzo ; dos Santos, Vanessa Rodrigues ; Souza, Amanda Caselato Andolfatto ; Ribeiro, Lucas da Silva ; de Camargo, Emerson Rodrigues ; Prakki, Anuradha ; Duque, Cristiane

• Document type: Journal article
• Source: JOURNAL OF FUNCTIONAL BIOMATERIALS; v. 14, n. 3, p. 17-pg., 2023-03-01.

Abstract

This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules' deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells. (AU)

• FAPESP's process: 17/10940-1 - Flavonoids combinations in thermosensitive hidrogels as proposals of endodontic medications for young permanent teeth
• Grantee: Cristiane Duque
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/12871-0 - Modification of the temperature transition profile of thermosensitive nanocomposites
• Grantee: Lucas da Silva Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)