Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons

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Godoi-Jr, Ederaldo P. ; Bronzato, Juliana D. ; Francisco, Priscila A. ; Bicego-Pereira, Eloa C. ; Lopes, Erica M. ; Passini, Maicon R. Z. ; de-Jesus-Soares, Adriana ; Almeida, Jose F. A. ; Marciano, Marina A. ; Ferraz, Caio C. R. ; Gomes, Brenda P. F. A.

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 27, n. 5, p. 16-pg., 2023-03-31.

Abstract

ObjectiveThe objective of this study was to evaluate the microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections evidenced by the presence of chronic apical periodontitis (G1) or for prosthetic reasons, without clinical and radiographic signs of endodontic reinfection (G2).MethodsMicrobiological samples were collected from thirty teeth (N=30) out of which 15 were indicated for retreatment due to the presence of chronic apical periodontitis (G1) and 15 were indicated exclusively for prosthetic reasons (G2). Samples were collected from root canals before (S1), after chemomechanical preparation (S2), and after 30 days of intracanal medication composed of calcium hydroxide and 2% chlorhexidine gel (S3). The molecular analysis was performed using Nested-PCR for the detection of 17 bacterial species. The efficacy of each stage of the retreatment in reducing the microbial load was verified by counting colony-forming units (CFU). The statistical analysis considered a significance level of 5%.ResultsThe results showed that bacteria were detected in 100% of the cases in S1, in both groups, by Nested PCR. The most frequently found species in S1 in both groups were Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Parvimonas micra. The microbial load of G1 was higher than G2 in the initial samples (S1). Endodontic retreatment was effective in reducing the microbial load in G1 and G2. Statistically significant associations were found between specific bacteria and clinical signs and symptoms.ConclusionIt was concluded that the microbial community present in the root canal of teeth indicated for endodontic retreatment is mixed and heterogeneous. G1 and G2 differ in the number of species per case and microbial load. (AU)

• FAPESP's process: 19/14448-0 - Microbiomes and immunobiological aspects in endodontic infections
• Grantee: Erica Mendes Lopes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/16516-7 - Investigation of the microbiological content, matrix metalloproteinase expression and antimicrobial susceptibility of specific bacteria in endodontic failure cases
• Grantee: Priscila Amanda Francisco
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/25090-3 - Microbiological and fadA family analysis in root canals and periapical lesions of teeth with secondary infection and their relation with the levels of proinflammatory cytokines and MMPs
• Grantee: Juliana Delatorre Bronzato
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/13871-6 - Decoding the microbiota correlations of teeth indicated for endodontic retreatment and the complex interrelationship between mobiloma and resistome in oral site metagenomes
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/14570-0 - INFLUENCE OF THE INFECTION TIME ON THE MICROBIOLOGICAL (BACTERIA AND FUNGI), CYTOTOXIC AND INFLAMMATORY PROFILE OF THE MICROBIAL COMMUNITY OF SECONDARY/PERSISTENT ENDODONTIC INFECTIONS
• Grantee: Ederaldo Pietrafesa de Godoi Junior
• Support Opportunities: Scholarships in Brazil - Doctorate