Cytotoxicity analysis of vertically aligned multi-walled carbon nanotubes by colorimetric assays

Author(s)

Lobo, A. O. ; Corat, M. A. F. ; Antunes, E. F. ; Palma, M. B. S. ; Pacheco-Soares, C. ; Corat, E. J.

• Document type: Journal article
• Source: Synthetic Metals; v. 159, n. 21-22, p. 2-pg., 2009-11-01.

Abstract

A new methodology to evaluate the acute cytotoxicity of vertically aligned multi-walled carbon nanotube (VACNT) scaffolds was presented. A comparison between three different colorimetric assays was performed: (i) lactate dehydrogenase,(ii)2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide, and (iii) neutral red. The results confirmed that a simple removing of VACNT scaffolds from the cell culture before the colorimetric assays, is necessary to obtain a high level of cell viability. (C) 2009 Published by Elsevier B.V. (AU)

• FAPESP's process: 08/11642-5 - Development of structures based on carbon nanotubes, bioresorbable polymers and carbon fibers for studies with regenerative cells and hematopoietic stem cells
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Scholarships in Brazil - Doctorate