Salmonella enterica Typhimurium: establishment of cultivation condition on shake flask and flagellin isolation strategy by using tangencial ultrafiltration

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Author(s):	Oliveira, B. H. ; Silva, M. R. ; Braga, C. J. M. ; Massis, L. M. ; Ferreira, L. C. S. ; Takagi, M. E. Sbrogio-Almeida M. ; MendezVilas, A Total Authors: 7
Document type:	Journal article
Source:	MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS; v. N/A, p. 4-pg., 2011-01-01.
Abstract
Flagellin is a main protein present in the bacterial flagellum and its molecular mass is around 51.5 kDa. Its role as an adjuvant vaccine has been intensively studied and currently there is evidence to be an immunomodulador in situations of autoimmune diseases, co-administered with chemotherapy or as radioprotetor. This study focus on to establish the native flagellin production by Salmonella Typhimurium in shake flask and flagellin purification strategy by using tangencial ultrafiltration.. Results showed that flagellin can be released to the supernatant depend on the agitation speed, and tangencial ultrafiltration can be used as tool to purify this protein. (AU)