Functional sugar formulation from Bifidobacterium breve metabolites.

• Grant number: 23/04486-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2023
• End date: September 30, 2024
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Pedro de Oliva Neto
• Grantee: Grasiela de Almeida Campos
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

The use of probiotics has been growing, mainly with the increase in the consumption of fermented milk and its effects on the improvement of intestinal problems. However, a new category of "biotics" has recently been discovered, the post-biotic, which are the metabolites obtained from the cultivation of probiotic lactic acid bacteria and subsequent centrifugation. Postbiotics have advantages such as inhibition of pathogens, modulation of the defense system, absorption of calcium and iron, among others.The objective of this project is the production of Bifidobacterium breve postbiotic in formulated media, the evaluation and formulation of a new sweetener based on a mixture of commercial sugar with these postbiotics. Therefore, the Minimum Inhibitory Concentration (MIC) of the postbiotic (PB) of Bifidobacterium breve BB-03 Danisco (Madison, USA) will be determined in different formulations of culture medium derived from MRS (De Man, Rogosa and Sharpe, 1960 ). The ability of this product to inhibit "in vitro" the growth of the pathogen Salmonella enterica serovar typhimurium ATCC 14028, strain obtained from the Tropical Foundation "André Tosello", will also be evaluated. In addition to mixing a post-biotic with commercial sugar and assessing its stability in terms of odor and taste, the effectiveness of inhibition of this sugar on the growth of S. typhimurium in different proportions will also be tested in order to find the best mixture of these components. Tests will be performed by adding different concentrations of dry mixture (PB + sugar) to the pathogen's culture medium and analyzing its growth through turbidity.