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Evaluation of the Modulation Dynamics of the Human Intestinal Microbiota in vitro from Prebiotic Oligosaccharides obtained from Lignocellulosic Wastes

Grant number: 23/17900-6
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 29, 2024
Effective date (End): September 28, 2024
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Rosana Goldbeck Coelho
Grantee:Marcos Fellipe da Silva
Supervisor: Clarisse Salome Nobre Goncalves
Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Research place: Universidade do Minho (UMinho), Portugal  
Associated to the scholarship:21/09527-8 - Integrated bioprocess for obtaining cello-oligosaccharides and cellulose nanocrystals through ultrasound-assisted enzymatic hydrolysis from agroindustrial wastes, BP.DR


Lignocellulosic agro-industrial residues are considered materials of low added value, often discarded by industries, however, they have a high potential for application in the production of xylo-oligosaccharides (XOS) and cello-oligosaccharides (COS). These oligosaccharides are known as potential prebiotics, selectively stimulating the growth and activity of beneficial intestinal bacteria, such as those of the genera Lactobacillus spp. and Bifidobacterium spp., improving host health. Currently, recent research is still studying the prebiotic effect of XOS and COS, based on probiotic strains of Lactobacillus spp. and Bifidobacteria spp. isolated. To date, no studies have been found in the literature that studied the complete and integrated modulation of the intestinal microbiota, especially the human one, based on the supplementation of these oligosaccharides. Thus, the objective and differential of this study is to evaluate the effect of xylo-oligosaccharides and cello-oligosaccharides, obtained from the enzymatic hydrolysis of lignocellulosic agro-industrial residues, on the modulation dynamics of the human intestinal microbiota in vitro. Thus, these studies would be of great value and importance for the area of functional ingredient development. The XOS and COS will be obtained from the sequential enzymatic hydrolysis of lignocellulosic residues, which will be subjected to the simulated in vitro digestion protocol followed by in vitro human colonic fermentation, with the microbial population subsequently being characterized from DNA extraction and Reaction in Quantitative Real-Time Polymerase Chain, in addition to the carbohydrates and metabolites produced. In this way, the present work will contribute to the development of prebiotic functional ingredients from industrial waste, which can be applied in food formulations that promote greater healthiness for consumers, evaluating how these oligosaccharides act in the digestive system and human microbiota, in addition to seeking study the metabolites generated that have an important physiological role in the organism.

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