Detection of Bovine Leukemia Virus in Brains of Cattle with a Neurological Syndrome: Pathological and Molecular Studies

Author(s)

Ramos D'Angelino, Rubens Henrique ; Pituco, Edviges Maristela ; Cassaro Villalobos, Eliana Monteforte ; Harakava, Ricardo ; Gregori, Fabio ; Del Fava, Claudia

• Document type: Journal article
• Source: BIOMED RESEARCH INTERNATIONAL; v. 2013, p. 6-pg., 2013-01-01.

Abstract

Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME (P > 0.05). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions. (AU)

• FAPESP's process: 10/04692-6 - Sanitary surveillance of bovine encephalitis: bovine leukaemia virus as causative agent
• Grantee: Rubens Henrique Ramos D'Angelino
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/06513-4 - Sanitary surveillance of bovine encephalitis: bovine leukaemia virus as causative agent
• Grantee: Claudia Del Fava
• Support Opportunities: Regular Research Grants