Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle

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Monico-Neto, Marcos ; de Campos Giampa, Sara Quaglia ; Lee, Kil Sun ; de Melo, Camila Maria ; Souza, Helton de Sa ; Dattilo, Murilo ; Minali, Paulo Alexandre ; Santos Prado, Pedro Henrique ; Tufik, Sergio ; de Mello, Marco Tulio ; Moreira Antunes, Hanna Karen

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF ENDOCRINOLOGY; v. 2015, p. 6-pg., 2015-01-01.

Abstract

Objective. Describe multicompartmental changes in the fat and various muscle fiber types, as well as the hormonal profile and metabolic rate induced by SD in rats. Methods. Twenty adult male Wistar rats were equally distributed into two groups: experimental group (EG) and control group (CG). The EG was submitted to SD for 96 h. Blood levels of corticosterone (CORT), total testosterone (TESTO), insulin like growth factor-1 (IGF-1), and thyroid hormones (T3 and T4) were used to assess the catabolic environment. Muscle trophism was measured using a cross-sectional area of various muscles (glycolytic, mixed, and oxidative), and lipolysis was inferred by the weight of fat depots from various locations, such as subcutaneous, retroperitoneal, and epididymal. The metabolic rate was measured using oxygen consumption (VO2) measurement. Results. SD increased CORT levels and decreased TESTO, IGF-1, and T4. All fat depots were reduced in weight after SD. Glycolytic and mixed muscles showed atrophy, whereas atrophy was not observed in oxidative muscle. Conclusion. Our data suggest that glycolytic muscle fibers are more sensitive to atrophy than oxidative fibers during SD and that fat depots are reduced regardless of their location. (AU)

• FAPESP's process: 13/00152-5 - Effect of resistance exercise in cardiac and skeletal muscle of Wistar rats submitted to paradoxical sleep deprivation
• Grantee: Hanna Karen Moreira Antunes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 98/14303-3 - Center for Sleep Studies
• Grantee: Sergio Tufik
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/15962-7 - Effects of resistance exercise on trophic and atrophic patways in skeletal muscle of rats with paradoxal sleep deprivation
• Grantee: Marcos Mônico Neto
• Support Opportunities: Scholarships in Brazil - Master