Ultrasmall cationic superparamagnetic iron oxide nanoparticles as nontoxic and efficient MRI contrast agent and magnetic-targeting tool

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Uchiyama, Mayara Klimuk ; Toma, Sergio Hiroshi ; de Paula Rodrigues, Stephen Fernandes ; Borges Shimada, Ana Lucia ; Loiola, Rodrigo Azevedo ; Cervantes Rodriguez, Hernan Joel ; Oliveira, Pedro Vitoriano ; Luz, Maciel Santos ; Rabbani, Said Rahnamaye ; Toma, Henrique Eisi ; Poliselli Farsky, Sandra Helena ; Araki, Koiti

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 10, p. 16-pg., 2015-01-01.

Abstract

Fully dispersible, cationic ultrasmall (7 nm diameter) superparamagnetic iron oxide nanoparticles, exhibiting high relaxivity (178 mM(-1)s(-1) in 0.47 T) and no acute or subchronic toxicity in Wistar rats, were studied and their suitability as contrast agents for magnetic resonance imaging and material for development of new diagnostic and treatment tools demonstrated. After intravenous injection (10 mg/kg body weight), they circulated throughout the vascular system causing no microhemorrhage or thrombus, neither inflammatory processes at the mesentery vascular bed and hepatic sinusoids (leukocyte rolling, adhesion, or migration as evaluated by intravital microscopy), but having been spontaneously concentrated in the liver, spleen, and kidneys, they caused strong negative contrast. The nanoparticles are cleared from kidneys and bladder in few days, whereas the complete elimination from liver and spleen occurred only after 4 weeks. Ex vivo studies demonstrated that cationic ultrasmall superparamagnetic iron oxide nanoparticles caused no effects on hepatic and renal enzymes dosage as well as on leukocyte count. In addition, they were readily concentrated in rat thigh by a magnet showing its potential as magnetically targeted carriers of therapeutic and diagnostic agents. Summarizing, cationic ultrasmall superparamagnetic iron oxide nanoparticles are nontoxic and efficient magnetic resonance imaging contrast agents useful as platform for the development of new materials for application in theranostics. (AU)

• FAPESP's process: 11/09677-8 - Experimental study of exposure to PCB126 on induction of type II Diabetes Mellitus
• Grantee: Ana Lucia Borges Shimada
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/08584-6 - Supramolecular chemistry and nanotechnology
• Grantee: Henrique Eisi Toma
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/02438-8 - THERAPEUTIC EFFICACY OF INDOMETHACIN AND INDOMETHACIN ETHYL ESTER NANOCAPSULES IN THE CEREBRAL MICROCIRCULATION OF MICE: IN VIVO AND EX VIVO STUDIES
• Grantee: Stephen Fernandes de Paula Rodrigues
• Support Opportunities: Scholarships in Brazil - Post-Doctoral