"Aroeira" (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects

Author(s)

Machado, Alessandra Cury ; Souza, Leonardo Perez ; Saldanha, Luiz Leonardo ; Pieroni, Lais Goyos ; Matos, Adriana Arruda ; de Oliveira, Flavia Amadeu ; Vilegas, Wagner ; Damante, Carla Andreotti ; Dokkedal, Anne Ligia ; de Oliveira, Rodrigo Cardoso

• Document type: Journal article
• Source: Pharmaceutical Biology; v. 54, n. 11, p. 5-pg., 2016-11-01.

Abstract

Context: Aroeira [Myracrodruon urundeuva AllemAo (Anacardiaceae)] is a tree whose leaves have been studied for therapeutic purposes in medicine and dentistry.Objective: The study chemically identifies the leaf extract of aroeira and determines its effect on human gingival fibroblasts.Materials and methods: An 80% methanol leave extract was obtained by maceration and chemically identified through flow-injection analysis-electrospray ionization-ion trap-tandem mass spectrometry (FIA-ESI-IT-MSn). Cytotoxicity of the aroeira's methanol extract was evaluated in lineage of fibroblasts. Adherent cells were treated with different concentrations of aroeira's methanol extract in the medium: 0.1, 1, 10, 100 and 1000g/mL. Control cells were cultivated in the medium only. Analyses were done at 24, 48, 72 and 96h of culture by neutral red assay; and at 24, 48 and 96h by crystal violet assay.Results: FIA-ESI-IT-MS analysis determined the presence of compounds, for the first time in the species: quercetin-O-glucuronide and quercetin-O-deoxyhexose-O-glucose in the extract. On one hand, neutral red and crystal violet assay showed a reduction (to 50% up until 100%) of cellular viability of groups of 100 and 1000g/mL compared with control at 96h (p<0.05). On the other hand, lower concentrations (0.1; 1 and 10g/mL) of the extract were similar to that of the control at 96h (p<0.05), in general.Conclusions: In view of the results, we can conclude that the extract of aroeira presents tannins and flavonoids. Furthermore, the extract is capable of modulating the viability of human gingival fibroblasts according to its concentration. (AU)

• FAPESP's process: 11/22243-7 - Effect of aroeira extract on adherence and proliferation of fibroblasts
• Grantee: Patricia Fernandes Sartori
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/14039-8 - Effect of the extract of aroeira in the process of proliferation and mineralization of human osteoblasts in vitro
• Grantee: Adriana Arruda Matos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/02026-9 - Evaluations of aroeira"s extract influence in adhesion and proliferate of human fibroblast and MMP-2 and MMP-9 expression in vitro
• Grantee: Rodrigo Cardoso de Oliveira
• Support Opportunities: Regular Research Grants