A Perspective on Extracellular Vesicles Proteomics

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Author(s):	Rosa-Fernandes, Livia ; Rocha, Victoria Bombarda ; Carregari, Victor Corasolla ; Urbani, Andrea ; Palmisano, Giuseppe Total Authors: 5
Document type:	Journal article
Source:	RONTIERS IN CHEMISTR; v. 5, p. 19-pg., 2017-11-21.
Abstract
Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function. (AU)

FAPESP's process:	14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications
Grantee:	Giuseppe Palmisano
Support Opportunities:	Research Grants - Young Investigators Grants